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Molecular and Cellular Biochemistry

, Volume 234, Issue 1, pp 81–90 | Cite as

Differential role of hydrogen peroxide in UV-induced signal transduction

  • Min Ding
  • Jingxia Lia
  • Stephen S. Leonard
  • Xianglin Shi
  • Max Costa
  • Vincent Castranova
  • Val Vallyathan
  • Chuanshu Huang
Article

Abstract

The present study investigated the differential requirement of ROS in UV-induced activation of these pathways. Exposure of the mouse epidermal Cl41 cells to UV radiation led to generation of ROS as measured by electron spin resonance (ESR) and by H2O2 and O2•−; fluorescence staining assay. Treatment of cells with UV radiation or H2O2 also markedly activated Erks, JNKs, p38 kinase and led to increases in phosphorylation of Akt and p70S6k in mouse epidermal JB6 cells. The scavenging of UV-generated H2O2 by N-acety-L-cyteine (NAC, a general antioxidant) or catalase (a specific H2O2 inhibitor) inhibited UV-induced activation of JNKs, p38 kinase, Akt and p70S6k, while it did not show any inhibitory effects on Erks activation. Further, pretreatment of cells with sodium formate (an OH radical scavenger) or superoxide dismutase (O2•− radical scavenger) did not inhibit any of these pathways. These results demonstrate that H2O2 generation is required for UV-induced phosphorylation of Akt and p70S6k, and involved in activation of JNKs and p38 kinase, but not Erks.

oxidative stress antioxidants oxyradical scavengers MAP kinases 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Min Ding
    • 1
  • Jingxia Lia
    • 2
  • Stephen S. Leonard
    • 1
  • Xianglin Shi
    • 1
  • Max Costa
    • 2
  • Vincent Castranova
    • 1
  • Val Vallyathan
    • 1
  • Chuanshu Huang
    • 2
  1. 1.Health Effects Laboratory DivisionNational Institute for Occupational Safety and HealthMorgantownUSA
  2. 2.Nelson Institute of Environmental MedicineNew York University School of MedicineTuxedoUSA

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