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Euphytica

, Volume 125, Issue 2, pp 273–280 | Cite as

Simplified AFLP procedure as a tool for identification of strawberry cultivars and advanced breeding lines

  • Mirosław Tyrka
  • Piotr Dziadczyk
  • Jerzy A. Hortyński
Article

Abstract

DNA polymorphisms among 6 cultivars of Fragaria × ananassa (Duch.) and 13salinity tolerant clones were evaluated using simplified – PstI based Amplified Fragment Length Polymorphism procedure(PstIAFLP). Out of 129 amplification products obtained with 10 selective primers, 116 markers were polymorphic and could be used to distinguish all analyzed materials. Coordinate and cluster analyses revealed 2 main groups of clones and divided strawberry cultivars (CUL) and tested F1 hybrids of ‘Sweet Heart’(HYB). Mean genetic similarities in groups of cultivars and selected breeding lines (SEL) were significantly higher (0.722 and0.706, respectively, p < 0.05) than in group of SH hybrids (0.485). Results suggest that PstIAFLP method is sufficient for effective identification and useful for assessing the level of genetic diversity in strawberry cultivars and breeding lines. The presented method can bean alternative multilocus marker system to widespread RAPD method.

AFLP cultivar identification Fragaria × ananassa genetic diversity molecular markers strawberry 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Mirosław Tyrka
    • 1
  • Piotr Dziadczyk
    • 2
  • Jerzy A. Hortyński
    • 2
  1. 1.Institute of Genetics and Plants BreedingLublinPoland
  2. 2.Department of Genetics and Horticultural Plants BreedingLublinPoland

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