Water, Air, and Soil Pollution

, Volume 139, Issue 1–4, pp 1–13 | Cite as

Isolation and Optimization of PAH-Degradative Bacteria from Contaminated Soil for PAHs Bioremediation

  • J. W. C. WongEmail author
  • K. M. Lai
  • C. K. Wan
  • K. K. Ma
  • M. Fang


The objective of the present study is to isolate PAH-degradativebacteria from petroleum-contaminated soils and to optimize theirdegradative conditions including pH, glucose, nitrogen andphenanthrene concentrations required for bioremediationpurposes. Several bacterial strains were isolated throughenrichment and one strain, Burkholderia cocovenenans (BU-3)that was tentatively identified by the Biolog system,demonstrated a high removal rate of phenanthrene over otherstrains. More than 95% in 100 and 500 mg L-1 and 65% in1000 mg L-1 of phenanthrene contents was reduced in theculture media, respectively. Maximum rate of phenanthrene removalup to 4.2 mg hr-1 occurred in the culture containing 1000 mgL-1 phenanthrene. Media at a pH between 6.5 to 7.0 weremore favorable for the degradation of phenanthrene by BU-3.Although increasing glucose concentrations from 0.45 to 3 gL-1 resulted in a better bacterial growth of the isolatedbacteria, the degradation of phenanthrene was reducedsignificantly. Nitrogen supplement did not exert a significanteffect on bacterial growth and phenanthrene degradation. Theisolated Burkholderia cocovenenans BU-3 demonstrated to bea feasible strain for degradation of phenanthrene at a neutralpH, even up to a phenanthrene concentration of 1000 mg L-1.

bioremediation Burkholderia cocovenenans degradation glucose pH phenanthrene 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • J. W. C. Wong
    • 1
    Email author
  • K. M. Lai
    • 1
  • C. K. Wan
    • 1
  • K. K. Ma
    • 1
  • M. Fang
    • 1
  1. 1.Department of BiologyHong Kong Baptist UniversityKowloon TongHong Kong

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