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Microbiology

, Volume 71, Issue 3, pp 326–330 | Cite as

The Detection of Cyanobacterial Cells by a Non-Fluorescent in situ Hybridization Method

  • N. L. Bel'kova
  • H. M. Chung
  • T. S. Ahn
Article
  • 81 Downloads

Abstract

Seven cyanobacterial strains (Anabaena macrospora NIER10016, Oscillatoria sp. NIER10042, Microcystis aeruginosa NIER10015, M. ichtyoblabe NIER10025 and NIER10040, M. novacekii NIER10029, and M. wesenbergii NIER10068) were tested by a nonfluorescent in situ hybridization method using two specific horseradish peroxidase–labeled oligonucleotide probes and two chromogenic substrates. This approach was shown to be appropriate for the analysis of natural samples.

cyanobacteria in situ hybridization horseradish peroxidase 

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REFERENCES

  1. 1.
    Kondrat'eva, N.V. and Kovalenko, O.V., Kratkii opredelitel' vidov toksicheskikh sinezelenykh vodoroslei (A Concise Manual of Toxic Cyanobacteria), Kiev: Nauk. Dumka, 1975, p. 64.Google Scholar
  2. 2.
    Rouhianen, L., Sivonen, K., Buikema, W.J., and Haselkorn, R., Characterization of Toxin-producing Cyanobacteria by Using an Oligonucleotide Probe Containing a Tandemly Repeated Heptamer, J. Bacteriol., 1995, vol. 177, no. 20, pp. 6021–6026.Google Scholar
  3. 3.
    Garcia-Pichel, F., Prufert-Bebout, L., and Muyzer, G., Phenotypic and Phylogenetic Analysis Show Microcoleus chthonoplastes to Be a Cosmopolitan Cyanobacterium, Appl. Environ. Microbiol., 1996, vol. 62, no. 9, pp. 3284–3291.Google Scholar
  4. 4.
    Wilson, K.M., Schembri, M.A., Baker, P.D., and Saint, C.P., Molecular Characterization of the Toxic Cyanobacterium Cylindrospermopsis raborskii and Design of a Species-Specific PCR, Appl. Environ. Microbiol., vol. 66, no. 1, pp. 332–338.Google Scholar
  5. 5.
    Lyra, C., Hantula, J., Vainio, E., Rapala, J., Rouhiainen, L., and Sivonen, K., Characterization of Cyanobacteria by the SDS-PAGE of Whole-Cell Proteins and the PCR/RFLP of the 16S rRNA Genes, Arch. Microbiol., 1997, vol. 168, no. 3, pp. 176–184.Google Scholar
  6. 6.
    Smith, J.K., Parry, J.D., Day, J.C., and Smith, R.J., A PCR Technique Based on the Hip1 Interspersed Repetitive Sequence Distinguishes Cyanobacterial Species and Strains, Microbiology (UK), 1998, vol. 144, no. 10, pp. 2791–2801.Google Scholar
  7. 7.
    Neilan, B.A., Dittmann, E., Rouhiainen, L., Bass, A., Schaub, V., Sivonen, K., and Boerner, T., Nonribosomal Peptide Synthesis and Toxigenicity of Cyanobacteria, J. Bacteriol., 1999, vol. 181, no. 13, pp. 4089–4097.Google Scholar
  8. 8.
    Schoenhuber, W., Zarda, B., Eix, S., Rippka, R., Herdman, M., Ludwig, W., and Amann, R., In Situ Identification of Cyanobacteria with Horseradish Peroxidase-labeled, rRNA-targeted Oligonucleotide Probes, Appl. Environ. Microbiol., 1999, vol. 65, no. 3, pp. 1259–1267.Google Scholar
  9. 9.
    Rippka, R., Dernelles, J., Waterbury, J.B., Herdman, M., and Stanier, R.Y., Genetic Assignments, Strain Histories, and Properties of Pure Cultures of Cyanobacteria, J. Gen. Microbiol., 1979, vol. 111, pp. 1–61.Google Scholar
  10. 10.
    Amann, R.I., In situ Identification of Microorganisms by Whole Cell Hybridization with rRNA-targeted Nucleic Acid Probes, Molecular Microbial Ecology Manual, Akkermans, A.D.L., Ed., 1995, 3.3.6, pp. 1–15.Google Scholar
  11. 11.
    Zarda, B., Amann, R., Wallner, G., and Schleifer, K.-H., Identification of Single Bacterial Cells Using Digoxigenin-labelled, rRNA-targeted Oligonucleotides, J. Gen. Microbiol., 1991, vol. 137, pp. 2823–2830.Google Scholar
  12. 12.
    Amann, R.I., Zarda, B., Stahl, D.A., and Schleifer, K.-H., Identification of Individual Prokaryotic Cells by Using Enzyme-labelled, rRNA-targeted Oligonucleotide Probes, Appl. Environ. Microbiol., 1992, vol. 58, no. 9, pp. 3007–3011.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2002

Authors and Affiliations

  • N. L. Bel'kova
    • 1
  • H. M. Chung
    • 2
  • T. S. Ahn
    • 3
  1. 1.Limnological Institute, Siberian DivisionRussian Academy of SciencesIrkutskRussia
  2. 2.National Institute of Environmental ResearchSeoulSouth Korea
  3. 3.Kangwon National UniversityCh'unch'onSouth Korea

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