, Volume 125, Issue 2, pp 265–272 | Cite as

Segregation distortion in doubled haploid lines of barley (Hordeum vulgare L.) detected by simple sequence repeat (SSR) markers

  • Haitham Sayed
  • Hamed Kayyal
  • Luke Ramsey
  • Salvatore Ceccarelli
  • Michael Baum


A total of 147 simple sequence repeat (SSR) markers (including86 barley and 61 wheat microsatellite markers) were tested for their segregation in a doubled haploid (DH) and an F2 population of barley. The DH population consisted of 71 doubled haploid lines, developed from F1 plants of a cross between Tadmor and WI2291using isolated microspore culture technique. A genetic linkage map consisting of 43 microsatellite markers was constructed using the DH population. Particularly on chromosome 4H microsatellite markers showed distorted segregation ratios. Segregation of DH lines based on molecular markers were compared with segregation of 92 F2 lines from the same cross. The proportion of loci deviating from the expected monogenic segregation ratios in the DH population was significantly higher (19/43loci, 44%) than in the F2 population (7/43 loci, 16%). The deviation was biased towards the WI2291 parent alleles. In line with this observation, WI2291 was found to perform better than Tadmor in regenerating green plantlets with the isolated microspore-culture technique.

barley (Hordeum vulgaredoubled haploid (DH) microspore culture (MC) segregation distortion and simple sequencerepeat (SSR) 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Agache, S., J. Bachelier, J. De Buyser, Y. Henry & A. Rode, 1989. Genetic analysis of anther-culture response in wheat using aneuploid, chromosome substitution and translocation lines. Theor Appl Genet 77: 7–11.CrossRefGoogle Scholar
  2. Bassam, B.J., G. Caetano-Aandles & P.M. Gressholf, 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels, Anal Biochem 196: 80–83.PubMedCrossRefGoogle Scholar
  3. Becker, J. & M. Heun, 1995. Barley microsatellites: allele variation and mapping. Plant Mol Biol 27: 835–845.PubMedCrossRefGoogle Scholar
  4. Devaux, P., A. Kilian & A. Kleinhofs, 1995. Comparative mapping of the barley genome with male and female recombinationderived, doubled haploid populations. Mol Gen Genet 249: 600–608.PubMedCrossRefGoogle Scholar
  5. Foisset, N. & R. Delourme, 1996. Segregation distortion in androgenic plants. In: S.M. Jain, S.K. Sopory & R.E. Veilleux (Eds.) In Vitro Haploid Production in Higher Plants, pp. 189–201. Kluwer Academic Publishers, The Netherlands.Google Scholar
  6. Ghaemi, M., A. Sarrafi & R. Morris, 1995. Reciprocal substitutions analysis of embryo induction and plant regeneration from antherculture in wheat (Triticum aestivum L.) Genome 38: 158–165.Google Scholar
  7. Graner, A., A. Jahoor, J. Schondelmaier, H. Siedler, K. Pillen, G. Fischbeck, G. Wenzel & R.G. Herrmann, 1991. Construction of an RFLP map of barley. Theor Appl Genet 83: 250–256.CrossRefGoogle Scholar
  8. Henry, J. & J. De Buyser, 1985. Effect of the 1B/1R translocation on anther-culture ability in wheat (Triticum aestivum L.). Plant Cell Rep 4: 307–310.CrossRefGoogle Scholar
  9. Heun, M., A.E. Kennedy, J.A. Anderson, N.L.V. Lapitan, M.E. Sorrells & S.D. Tanksley, 1991. Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34: 437–447.Google Scholar
  10. Jähne, A., Lazzeri, P.A., M. Jäger-Gussen & H. Lörz, 1991. Plant regeneration from embryogenic cell suspensions derived from anther cultures of barley (Hordeum vulgare L.). Theor Appl Genet 82: 74–80.CrossRefGoogle Scholar
  11. Kintzios, S., R.M. Islam & G. Fischbeck, 1994. Distorted segregation for mildew resistance in doubled haploid lines of spring barley. Plant Breed 112: 248–251.CrossRefGoogle Scholar
  12. Kosambi, D.D. 1944. The estimation of map distances from recombination values. Ann Eugen 12: 172–175.Google Scholar
  13. Kuhlmann, U. & Foroughi-Wehr, 1989. Production of doubled haploid lines in frequencies sufficient for barley breeding programs. Plant Cell Reports 8: 78–81.CrossRefGoogle Scholar
  14. Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M. Daly, S. Lincoln & L. Newburg, 1987. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genetics 1: 174–181.Google Scholar
  15. Lashermes, P., G. Engin & G. Ortiz-Ferrara, 1991. Anther culture of wheat (Triticum aestivum) adapted to dry areas of West Asia and North Africa. J Genet & Breed 45: 33–38.Google Scholar
  16. Liu, Z.W., R.M. Biyashev & M.A. Saghai Maroof, 1996. Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor Appl Genet 93: 869–876.Google Scholar
  17. Logue, S.L., C. Oti-Boateng, A. Karakousis, J.M. Kretschmer, S. Manning, R.C.M. Lance & P.J. Langridge, 1995. Segregation analysis of DNA markers in anther culture-derived populations of barley (Hordeum vulgare L.) In: Proc 7th Aus Barley Tech Symp. Perth WA, pp. 210–217.Google Scholar
  18. Manninen, O.M., 2000. Associations between anther-culture response and molecular markers on chromosomes 2H, 3H and 4H of barley (Hordeum vulgare L.) Theor Appl Genet 100: 57–62.CrossRefGoogle Scholar
  19. Mather, K., 1957. The Measurement of Linkage in Heredity. pp. 1-149.Google Scholar
  20. Morgante, M, A. Rafalski, P. Bidle, S. Tingey & A.M. Oliveri, 1994. Genetic mapping and variability of seven soybean simple sequence repeat loci. Genome 37: 763–769.PubMedGoogle Scholar
  21. Patel, J.D., E. Reinbergs & S.O. Fejer, 1985. Recurrent selection in doubled haploid populations of barley (Hordeum vulgare L.). Can J Genet Cytol 27: 172–177.Google Scholar
  22. Pickering, R.A. & P. Devaux, 1992. Haploid production: approaches and use in plant breeding, In: P. Shewry (Ed.), Biotechnology in Agriculture No. 5, Barley: Genetics, Biochemistry, Molecular Biology and Biotechnology, pp. 519–547.Google Scholar
  23. Qi, X., P. Stam & P. Lindhout, 1998. Use of locus-specific AFLP markers to construct a high-density molecular map in barley. Theor Appl Genet 96: 376–384.CrossRefGoogle Scholar
  24. Ramsay, L., M. Macaulay, S. degli Ivanissevich, K. MacLean, L. Cardle, J. Fuller, K.J. Edwards, S. Tuvesson, M. Morgante, A. Massari, E. Maestri, N. Marmiroli, T. Sjakste, M. Ganal, W. Powell & R. Waugh, 2000. A simple sequence repeat-based linkage map of barley. Genetics 156: 1997–2005.PubMedGoogle Scholar
  25. Russell, J., J. Fuller, G. Young, B. Thomas, G. Taramino, M. Macaulay, R. Waugh & W. Powell, 1997. Discriminating between barley genotypes using microsatellite markers. Genome 40: 442–450.PubMedGoogle Scholar
  26. Röder, M.S., J. Plaschke, König, A. Börner, M.E. Sorrells, S.D. Tanksley & M.W. Ganal, 1995. Abundance variability and chromosomal location of microsatellites in wheat. Mol Gen Genet 246: 327–333.PubMedCrossRefGoogle Scholar
  27. Saghai Maroof, M.A., K.M. Soliman, R.A. Jorgenson & R.W. Allard, 1984. Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81: 8014–8018.PubMedCrossRefGoogle Scholar
  28. Sibikeeva, Y.E. & S.N. Sibikeev, 1996. Genetic analysis of antherculture response in wheat carring alien translocations. Theor Appl Genet 92: 782–785.CrossRefGoogle Scholar
  29. Snape, J.W., 1996. QTL strategies in cereals. Plant Genome IV. Scherago Int, NY, pp. 13.Google Scholar
  30. Stam, P. & J.W. Van Ooijen, 1995. JoinMap version 2.0: software for the calculation of genetic linkage maps. CPRO-DLO, Wageningen.Google Scholar
  31. Steffenson, B.J., Y. Jin, B.G. Rossnagel, J.B. Rasmussen & K. Kao, 1995. Genetics of multiple disease resistance in a doubledhaploid population of barley. Plant Breed 114: 50–54.CrossRefGoogle Scholar
  32. Thompson, D.M., K. Chalmers, R. Waugh, B.P. Forster, W.T.B. Thomas, P.D.S. Caligari & W. Powell, 1991. The inheritance of genetic markers in microspore-derived plants of barley (Hordeum vulgare L). Theor Appl Genet 81: 487–492.CrossRefGoogle Scholar
  33. Xu, Y., L. Zhu, J. Xiao, N. Huang & S.R. McCouch, 1997. Chromosomal regions associated with segregation distortion of molecular markers in F2, backcross, doubled haploid and recombinant inbred populations in rice (Oryza sativa L.).Mol Gen Genet 253: 535–545.PubMedCrossRefGoogle Scholar
  34. Zamir, D. & Y. Tadmor, 1986. Unequal segregation of nuclear genes in plants. Bot Gaz 147(3): 355–358.CrossRefGoogle Scholar
  35. Zivy, M., P. Devaux, J. Blaisonneau, R. Jean & H. Thiellement, 1992. Segregation distortion and linkage studies in microsporederived doubled-haploid lines of Hordeum vulgare L. Theor Appl Genet 83: 919–924.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Haitham Sayed
    • 1
    • 2
  • Hamed Kayyal
    • 2
  • Luke Ramsey
    • 3
  • Salvatore Ceccarelli
    • 1
  • Michael Baum
    • 1
  1. 1.ICARDAThe International Center for Agricultural Research in the Dry Areas (AleppoSyria
  2. 2.Faculty of AgricultureDamascus UniversityDamascusSyria
  3. 3.Scottish Crop Research InstituteDundeeScotland

Personalised recommendations