Biodiversity & Conservation

, Volume 11, Issue 6, pp 1081–1089 | Cite as

Micropropagation of endangered endemic Brazilian bromeliad Cryptanthus sinuosus (L.B. Smith) for in vitro preservation

  • R. Arrabal
  • F. Amancio
  • L.A. Carneiro
  • L.J. Neves
  • E. Mansur


An efficient liquid culture system for plant regeneration from leaflessstem–root axes of Cryptanthus sinuosus L. B. Smith(Bromeliaceae) was established. High regeneration rates (93%) were achieved inMurashige and Skoog's medium without growth regulators. Whole plants wereobtained in a single-step procedure, resulting in the production of 25.3± 3.6 plants/explant after 6 months of culture. Incubationof plant material at 35 ± 3 °C resulted in an increaseof 60% in the regeneration efficiency compared with tissues incubated at 28± 2 °C. Moreover, after 5–6 sub-cultures in thesame medium, the axes originated bud clusters that could be continuouslymultiplied and gave rise to 19.4 ± 3.2 whole plants per gram of freshmatter. It was estimated that the liquid culture system described is potentiallyable to produce about 4500 plants/explant/year. Rates of 98% acclimatizationwere achieved. The use of plants produced following this method for populationreinforcement and for in vitro preservation programs ofendangered populations is suggested.

Endangered populations Histological analysis In vitro preservation In vitro regeneration Liquid culture 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • R. Arrabal
    • 1
  • F. Amancio
    • 1
  • L.A. Carneiro
    • 1
  • L.J. Neves
    • 2
  • E. Mansur
    • 1
  1. 1.Laboratório de Micropropagação e Transformaoçã de Plantas, I. Biologia, DBCGUniversidade do Estado do Rio de JaneiroRio de Janeiro, RJBrazil
  2. 2.Laboratório de Anatomia VegetalUniversidade Federal do Rio de JaneiroMuseu Nacional do Rio de JaneiroBrazil

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