Biotechnology Letters

, Volume 24, Issue 12, pp 1031–1036 | Cite as

Hairy root cultures of Anethum graveolens (dill): establishment, growth, time-course study of their essential oil and its comparison with parent plant oils

  • Pedro A.G. Santos
  • A. Cristina Figueiredo
  • Pedro M.L. Lourenço
  • José G. Barroso
  • Luis G. Pedro
  • M. Margarida Oliveira
  • Jan Schripsema
  • Stanley G. Deans
  • Johannes J.C. Scheffer
Article

Abstract

Transformed root cultures of Anethum graveolens were induced by inoculation of aseptically grown seedlings with Agrobacterium rhizogenes carrying plasmid pRi 1855. The main component of the essential oils from the fruits and from the roots of the parent plant was carvone, whereas α-phellandrene and apiole were dominant in the oil from, respectively, the aerial parts and the hairy roots. The essential oils from the fruits, aerial parts and roots of the parent plant were at 2%, 0.3% and 0.06% (v/w), respectively, but only 0.02% (v/w) in the hairy root cultures. Growth of the hairy root cultures reached 600 mg dry wt/50 ml medium after 50 days. The essential oil composition did not change significantly during their growth.

Agrobacterium rhizogenes Anethum graveolens Apiaceae essential oil hairy root cultures 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Pedro A.G. Santos
    • 1
  • A. Cristina Figueiredo
    • 1
  • Pedro M.L. Lourenço
    • 1
  • José G. Barroso
    • 1
  • Luis G. Pedro
    • 1
  • M. Margarida Oliveira
    • 2
  • Jan Schripsema
    • 3
  • Stanley G. Deans
    • 4
  • Johannes J.C. Scheffer
    • 5
  1. 1.Centro de Biotecnologia Vegetal, Faculdade de Cîencias de LisboaDepartamento de Biologia VegetalLisbonPortugal
  2. 2.ITQB/IBETQuinta do MarqûesOeirasPortugal
  3. 3.Setor de Quimica de Produtos Naturais, LCQUI/CCTUniversidade Estadual do Norte FluminenseCampo dos Goytacazes, Rio de JaneiroBrasil
  4. 4.Department of Food Science & Technology, SACAuchincruiveAyrScotland, UK
  5. 5.Division of Pharmacognosy, LACDRLeiden University, Gorlaeus LaboratoriesLeidenThe Netherlands

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