Biotechnology Letters

, Volume 24, Issue 11, pp 857–860 | Cite as

A caveat for the use of log P values for the assessment of the biocompatibility of organic solvents

  • Mateja Pogorevc
  • Hartmut Stecher
  • Kurt Faber
Article

Abstract

The degree of enzyme deactivation for lipases from Candida rugosa and Pseudomonas sp., hydroxynitrile lyase and mandelate racemase upon exposure to organic solvents can be correlated to their respective partition coefficients (log P values). However, three unexpected results were obtained: (1) the deactivation exerted by protic solvents, e.g., methanol, is severely underestimated; (2) little deactivation by an organic solvent cannot neccessarily be correlated to catalytic activity in this medium, and (3) in contrast to other enzymes, hydroxynitrile lyase is exceptionally stable towards deactivation by DMF.

enzyme deactivation hydroxynitrile lyase lipase log P mandelate racemase 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Mateja Pogorevc
    • 1
  • Hartmut Stecher
    • 1
  • Kurt Faber
    • 1
  1. 1.Department of Chemistry, Organic & Bioorganic ChemistryUniversity of GrazGrazAustria

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