Biotechnology Letters

, Volume 24, Issue 11, pp 879–883 | Cite as

Enhanced detoxification of organophosphates using recombinant Escherichia coli with co-expression of organophosphorus hydrolase and bacterial hemoglobin

  • Dong Gyun Kang
  • Jaoon Y.H. Kim
  • Hyung Joon Cha


A significantly improved, recombinant Escherichia coli has been developed to degrade the toxic organophosphorus compound, Paraoxon, to non-toxic materials by co-expression of organophosphorus hydrolase (OPH) under trc promoter and Vitreoscilla hemoglobin (VHb) under O2dependent nar promoter. VHb-expressing whole cells had significant enhancement of OPH activity (48%, 18.7 vs. 27.8 unit l−1) and bioconversion efficiency Vmax/Km (44%, 0.14 vs. 0.2 min−1) compared to VHb-free system.

bacterial hemoglobin organophosphate organophosphorus hydrolase recombinant Escherichia coli whole cell bioconversion 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Dong Gyun Kang
    • 1
  • Jaoon Y.H. Kim
    • 1
  • Hyung Joon Cha
    • 1
  1. 1.Department of Chemical Engineering & Division of Molecular and Life SciencesPohang University of Science and TechnologyPohangKorea

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