The Content and Chain Length of Polyphosphates from Vacuoles of Saccharomyces cerevisiae VKM Y-1173
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- Trilisenko, L.V., Vagabov, V.M. & Kulaev, I.S. Biochemistry (Moscow) (2002) 67: 592. doi:10.1023/A:1015510631271
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The content of inorganic polyphosphates (polyP) in vacuoles of the yeast Saccharomyces cerevisiae is ∼15% of the total cellular polyP. Over 80% of the vacuole polyP are in an acid-soluble fraction. It was first established by 31P-NMR spectroscopy that a polymeric degree (n) of two subfractions obtained by precipitation with Ba2+ in succession at pH 4.5 and 8.2 was approximately 20 ± 5 and 5 ± 2 residues of ortho-phosphoric acid, respectively. Under a deficit of phosphate (Pi) in the cultivation medium, the polyP content in vacuoles decreased ∼7-fold with the same drastic reduction of their content in the cell. Unlike intact yeast cells, where polyP overcompensation is observed after their transfer from phosphate-free to phosphate-containing medium, the vacuoles do not show this effect. The data indicate the occurrence of special regulatory mechanisms of polyP synthesis in vacuoles differing from those in the whole cell.
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