Biochemistry (Moscow)

, Volume 67, Issue 5, pp 592–596

The Content and Chain Length of Polyphosphates from Vacuoles of Saccharomyces cerevisiae VKM Y-1173

Article

DOI: 10.1023/A:1015510631271

Cite this article as:
Trilisenko, L.V., Vagabov, V.M. & Kulaev, I.S. Biochemistry (Moscow) (2002) 67: 592. doi:10.1023/A:1015510631271

Abstract

The content of inorganic polyphosphates (polyP) in vacuoles of the yeast Saccharomyces cerevisiae is ∼15% of the total cellular polyP. Over 80% of the vacuole polyP are in an acid-soluble fraction. It was first established by 31P-NMR spectroscopy that a polymeric degree (n) of two subfractions obtained by precipitation with Ba2+ in succession at pH 4.5 and 8.2 was approximately 20 ± 5 and 5 ± 2 residues of ortho-phosphoric acid, respectively. Under a deficit of phosphate (Pi) in the cultivation medium, the polyP content in vacuoles decreased ∼7-fold with the same drastic reduction of their content in the cell. Unlike intact yeast cells, where polyP overcompensation is observed after their transfer from phosphate-free to phosphate-containing medium, the vacuoles do not show this effect. The data indicate the occurrence of special regulatory mechanisms of polyP synthesis in vacuoles differing from those in the whole cell.

yeast protoplasts vacuoles overcompensation polyphosphates exopolyphosphatase 

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Copyright information

© MAIK “Nauka/Interperiodica” 2002

Authors and Affiliations

  • L. V. Trilisenko
    • 1
  • V. M. Vagabov
    • 1
  • I. S. Kulaev
    • 1
  1. 1.Skryabin Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchino, Moscow RegionRussia

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