, 154:41 | Cite as

A Stachybotrys chartarum isolate from soybean

  • Shuxian Li
  • Glen L. Hartman
  • Bruce B. Jarvis
  • Heekyung Tak


As part of our effort to investigate fungi associated with soybean roots, Stachybotrys chartarum was isolated from soybean root lesions. Since this fungus has not been reported to cause a disease of soybean, the objectives were to identify and characterize this fungus using biological, chemical, and molecular approaches. Fungal morphology was examined using light and environmental scanning electron microscopy. Phialides bearing conidia arose from determinate, macronematous, dark olivaceous conidiophores. The phialides were obovate or ellipsoidal in whorls. Conidia were unicellular, round or ellipsoidal, 5–13 × 4–7 μm, initially hyaline with smooth walls then dark brown to black and rough-walled when mature. Radial growth of the fungus on cornmeal, oatmeal and potato dextrose agar was 38, 47, and 33 mm in diam., respectively, after 10 days at 25 °C. Pathogenicity was performed using sorghum grain colonized by S. chartarum placed below sown soybean seeds in a soil : sand (1 : 1) steam-pasteurized mix. Three weeks after inoculation, root lesions ranged from 7 to 25 mm long. The fungus was reisolated from soybean root lesions and was reidentified as S. chartarum. Biochemical analysis indicated that this soybean isolate produced satratoxins G and H along with roridin L-2, as well as the spircyclic lactones and lactams in rice culture. PCR using a S. chartarum-specific primer StacR3 and IT51 amplified a 198-bp DNA fragment from the total genomic DNA. The DNA sequence of the ITS region was 100% identical to the S. chartarum strain ATCC 9182, one nucleotide mismatch with S. chartarum strain UAMH 7900, and differed from all published sequences of 12 other species of Stachybotrys and 2 species of Memnoniella in GenBank with genetic divergence ranging from 5.26 to 9.98%. This molecular evidence further supports the identification of S. chartarum isolated from soybean root lesions.

DNA sequence microscopy mycotoxin PCR soybean pathogen Stachybotrys chartarum 


  1. 1.
    Forgacs J. Stachybotryotoxicosis. In: Kadis S, Geigler A, Ajl SJ, eds. Microbial Toxins. New York: Academic Press, 1972; 95–128.Google Scholar
  2. 2.
    Harrach B, Mirocha CJ, Pathre SV, Palyusik M. Macrocyclic trichothecene toxins produced by a strain of Stachybotrys atra from Hungary. Appl Environ Microbiol 1981; 41: 1428–1432.PubMedGoogle Scholar
  3. 3.
    Hendry KM, Cole EC. A review of mycotoxins in indoor air. J Toxicol Environ Health 1993; 38: 183–198.PubMedCrossRefGoogle Scholar
  4. 4.
    Hintikka EL. Human stachybotryotoxicosis. In: Wyllie TD, Morehouse LG, eds. Mycotoxic Fungi, Mycotoxins, Mycotoxicoses. New York: Marcel Dekker, 1978; 87–89.Google Scholar
  5. 5.
    Jarvis, BB, Salemme J, Morais A. Stachybotrys toxins. Nat Toxins 1995; 3: 10–16.PubMedGoogle Scholar
  6. 6.
    Fung F, Clark R, Williams S. Stachybotrys, a mycotoxinproducing fungus of increasing toxicologic importance. J Toxicol Clin Toxicol 1998; 36: 79–86.PubMedCrossRefGoogle Scholar
  7. 7.
    Hinkley, SF, Mazzola, EP, Fettinger, JC, Lam, Y-F, Jarvis, BB. Atranones AG: A unique series of metabolites from the toxigenic mold Stachybotrys chartarum. Phytochemistry 2000; 55: 663–673.PubMedCrossRefGoogle Scholar
  8. 8.
    Jarvis, BB, Hinkley, SF, Nielsen, KF. Stachybotrys: An unusual mold associated with water-damaged buildings. Mycotoxin Res 2000; 16A: 105–108.CrossRefGoogle Scholar
  9. 9.
    Jarvis BB, Sorenson WG, Hintikka EL, Nikulin M, Zhou Y, Jiang J, Wang S, Hinkley S, Etzel RA, Dearborn D. Study of toxin production by isolates of Stachybotrys chartarum and Memnoniella echinata isolated during a study of pulmonary hemosiderosis in infants. Appl Environ Microbiol 1998; 64: 3620–3625.PubMedGoogle Scholar
  10. 10.
    Johanning E, Biagini R, Hull D, Morey P, Jarvis B, Landsbergis P. Health and immunology study following exposure to toxigenic fungi (Stachybotrys chartarum) in a water-damaged office environment. Int Arch Occup Environ Health 1996; 68: 207–218.PubMedGoogle Scholar
  11. 11.
    Dearborn DG, Yike, I, Sorenson, WG, Miller, M J, Etzel, RA. Overview of an investigation into pulmonary hemorrhage among infants in Clevel and Ohio. Environ. Health Persp. Suppl. 1999; 3, 107: 495–499.Google Scholar
  12. 12.
    Tsai, SM, Yang, CS, Heinsohn, P. Comparative studies of fungal media for recovery of Stachybotrys chartarum from environmental samples. In: E Johanning and CS Yang, eds. Bioaerosols, Fungi, and Mycotoxins: Health Effects, Assessment, Prevention, and Control. Albany, New York: Boyd Printing, 1999; 330–334.Google Scholar
  13. 13.
    Haugland RA, Heckman JL. Identification of putative sequence specific PCR primers for detection of the toxigenic fungal species Stachybotrys chartarum. Mol Cell Probes 1998; 12: 387–396.PubMedCrossRefGoogle Scholar
  14. 14.
    Sadaba RB, Vrijmoed LLP, Jones EBG, Hodgkiss IJ. Observations on vertical distribution of fungi associated with standing senescent Acanthus ilicifolius stems at Mai Po Mangrove, Hong Kong. Hydrobiologia 1995; 295: 119–126.CrossRefGoogle Scholar
  15. 15.
    Nejat SA, Ershad D. An investigation on mycoflora of barley seeds in Iran. Iranian J Plant Pathology 1994; 30: 23–28.Google Scholar
  16. 16.
    Catania M, Hladki A, Schiavone MM, Ramallo JC. Contribution to the study of the microflora in Nicotiana tabacum L. var. Burley (Tucuman, Argentina) Lilloa. 1998;. 39: 109–113.Google Scholar
  17. 17.
    Pathak, PD. A leaf spot disease of Crotolaria juncea L., caused by Stachybotrys atra Corda. Cuur Sci 1976; 45: 567.Google Scholar
  18. 18.
    Li S, Hartman GL. First Report of Stachybotrys chartarum causing soybean root rot. Plant Disease 2000; 84: 100.Google Scholar
  19. 19.
    Huang YH, Hartman GL. A semi-selective medium for detecting Fusarium solani, the causal organism of soybean sudden death syndrome. (Abstr.). Phytopathology 1996; 86: S12.Google Scholar
  20. 20.
    Huang, YH, Hartman GL. Reaction of selected soybean genotypes to isolates of Fusarium solani f. sp. glycines and their culture filtrates. Plant Dis 1998; 82: 999–1002.Google Scholar
  21. 21.
    Li S, Tam YK, Hartman GL. Molecular differentiation of Fusarium solani f. sp. glycines from other F. solani based on mitochondrial small subunit rDNA sequences. Phytopathology 2000; 90: 491–497.PubMedGoogle Scholar
  22. 22.
    Li S, Cullen D, Hjort M, Spears R, Andrews JH. Development of an oligonucleotide probe for Aureobasidium pullulans based on the small subunit rRNA gene. Appl Environ Microbiol 1996; 62: 1514–1518.PubMedGoogle Scholar
  23. 23.
    Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22: 4673–4680.PubMedGoogle Scholar
  24. 24.
    Hinkley SF, Jarvis BB. Method for Stachybotrys Toxins. In: Trucksess MW, Pohland AE. eds. Methods in Molecular Biology: The Mycotoxin Protocols. Vol. 157. Humana Press, 2000: 173–194.Google Scholar
  25. 25.
    Rayner RW. A Mycological Colour Chart. Commonw. Mycol. Inst. and British Mycol. Soci., Kew, Surrey, England, 1970.Google Scholar
  26. 26.
    Jong SC, Davis EE. Contribution to the knowledge of Stachybotrys and Memnoniella in culture. Mycotaxon 1976; 3: 409–485.Google Scholar
  27. 27.
    Cutler H, Jarvis BB. Preliminary observations on the effects of macrocyclic trichothecenes on plant growth. Exper Environ Bot 1985; 25: 115–128.CrossRefGoogle Scholar
  28. 28.
    Kang Z, Buchenauer H. Immunocytochemical localization of fusarium toxins in infected wheat spikes by Fusarium culmorum. Physiol Mol Plant Pathology 1999; 55: 275–288.CrossRefGoogle Scholar
  29. 29.
    Mesterhazy A, Bartok T, Mirocha CG, Komoroczy R. Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breeding 1999; 118: 97–110.CrossRefGoogle Scholar
  30. 30.
    Nikulin M, Pasanen, A-L., Berg, S., Hintikka, E-L. Stachybotrys atra growth and toxin production in some building materials and fodder under different relative humidities. Appl Environ Microbiol 1994; 60: 3421–3424.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Shuxian Li
    • 1
  • Glen L. Hartman
    • 1
    • 2
  • Bruce B. Jarvis
    • 3
  • Heekyung Tak
    • 3
  1. 1.National Soybean Research Center (NSRC), Department of Crop SciencesUniversity of IllinoisUrbanaUSA
  2. 2.USDA/ARS, NSRCUniversity of IllinoisUrbanaUSA
  3. 3.Department of Chemistry & Biochemistry; Joint Institute For Food Safety & Applied NutritionUniversity of MarylandCollege ParkUSA

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