Abstract
A micropropagation protocol of Bupleurum fruticosumL. was developed, in order to obtain a great number of plants for the production of secondary metabolites. The combination of 1.0 mg l−1 indole-3-acetic acid and 1.5 mg l−1 6-benzyladenine added to Murashige–Skoog medium resulted in the best multiplication. Root formation gave the same results in hormone-free medium and in the medium to which various concentrations of 1-naphthaleneacetic acid had been added. In both the multiplication and the rooting phase, 2, 5, 10 and 20 μg l−1 triacontanol were applied. After 4 weeks of culture, the number of shoots and nodes and the fresh weight were measured in the multiplication phase. Root number, shoot length, node number and fresh weight were determined in the root induction phase, while chlorophyll content was measured in both phases. In the multiplication phase 2 μg l−1 triacontanol was found to be the optimal concentration, the same as was the case in the rooting phase, except for the production of epigeous structures, for which the optimal concentration was 10 μg l−1.
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References
Burnat E (1897) Monographie des Buplévres des Alpes Maritimes. Georg & Co., Libraires - Editeurs, Lyon
David C (1901) Etude anatomique du genre Bupleurum. PhD Thesis, Lans- le-Saunier, Paris
Fiori A (1969) Flora d'Italia. 2: 217, Edagricole, Bologna
Giamperi L, Ricci D, Fraternale D, Manunta A & Tabacchi R (1998) The essential oil from Bupleurum fruticosum L. of the Cyrenaica Region of Eastern Libya and the problem of Bupleurol. J. Essent. Oil Res. 10: 369–374
Guinea MC, Parellada J, Lacaille-Dubois MA & Wagner H (1994) Biologically active triterpene saponins from Bupleurum fruticosum. Planta Med. 60: 163–164
Hangarter R, Ries SK & Carlson P (1978) Effect of triacontanol on plant cell cultures in vitro. Plant Physiol. 61: 855–857
Lorente I, Ocete MA, Zarzuelo A, Cabo MM & Jimenez J (1989) Bioactivity of the essential oil of Bupleurum fruticosum. J. Nat. Prod. 52: 267–272
Ma FW, Wang JC & Rong W (1990) Effects of plant growth regulators on in vitro propagation of apple cultivar Fuji. J. Fruit Sci. 7: 201–206
Manunta A, Tirillini B & Fraternale D (1992) Secretory tissues and essential oil composition of Bupleurum fruticosum L. J. Essent. Oil Res. 4: 461–466
Mészaros A, Bellon A, Pintér E & Horvath G (1999) Micropropagation of Lemon balm. Plant Cell, Tiss. Org. Cult. 57: 149–152
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–479
Peyron L & Roubaud M (1970) Sur une essence de Bupleurum fruticosum L. Plante Médicin Phytoth. 4: 172–175
Picci V, Atzei A & Manunta A (1974) About the content in essential oil in the officinal species of Sardinia: I. Morphosystematic and chemical notes on Bupleurum fruticosum L. Riv. Ital. EPPOS. 5: 1–6
Pignatti S (1982) Flora d'Italia. 2: 217, Edagricole, Bologna
Pistelli L, Bilia AR, Marsili A, De Tommasi N & Manunta A (1993) Triterpenoid saponins from Bupleurum fruticosum. J.Nat.Prod. 56: 240–244
Pistelli L, Bertoli A, Bilia AR & Morelli I (1996) Minor constituents from Bupleurum fruticosum roots. Phytochemistry 41: 1579–1582.
Tantos A, Mészaros A, Kissimon J, Horvath G & Farkas T (1999) The effect of triacontanol on micropropagation of balm, Melissa officinalis L. Plant Cell Rep. 19: 88–91
Yun CH & Kim HI (1986) Differences in callus formation from different explant sources and effect of plant growth regulators in plant regeneration in rice (Oryza sativa L.). Res. Rep. Rur. Dev. Admin. 1:113–116
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Fraternale, D., Giamperi, L., Ricci, D. et al. Micropropagation of Bupleurum fruticosum: The effect of triacontanol. Plant Cell, Tissue and Organ Culture 69, 135–140 (2002). https://doi.org/10.1023/A:1015245810053
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DOI: https://doi.org/10.1023/A:1015245810053