Biologia Plantarum

, Volume 45, Issue 2, pp 273–279

BTH-Induced Accumulation of Extracellular Proteins and Blackspot Disease in Rose

  • Y. Suo
  • D.W.M. Leung

DOI: 10.1023/A:1015161110058

Cite this article as:
Suo, Y. & Leung, D. Biologia Plantarum (2002) 45: 273. doi:10.1023/A:1015161110058


Treatment of rose shoots with 50 µM acibenzolar-S-methyl (BTH) resulted in increased protection against Diplocarpon rosae. This was accompanied by the induction and accumulation of a set of extracellular proteins as shown by SDS-PAGE and 2D-PAGE. Some of these proteins have been identified as PR-1, PR-2, PR-3 and PR-5 proteins by immunoblot analysis probed with tobacco antisera against PR-1c, PR-N, PR-Q and PR-S protein. Most of the extracellular proteins activated by BTH were also induced and found to accumulate in leaves upon infection with Diplocarpon rosae. However, their accumulation was much more pronounced in BTH-pretreated leaves than in water-pretreated leaves upon a challenge inoculation with D. rosae, particularly, the 15 kD PR-1, 36 and 37 kD PR-2 proteins. They may be more important in the expression of disease resistance.

acibenzolar-S-methyl Diplocarpon rosae induced resistance intercellular fluid pathogenesis-related proteins Rosa hybrida 

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Y. Suo
    • 1
  • D.W.M. Leung
    • 1
  1. 1.Department of Plant and Microbial SciencesUniversity of CanterburyChristchurch 1New Zealand

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