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Plant Growth Regulation

, Volume 36, Issue 2, pp 161–167 | Cite as

Effect of transition metals on stress, lipid peroxidation and antioxidant enzyme activities in tobacco cell cultures

  • Pablo BuenoEmail author
  • Abel Piqueras
Article

Abstract

to-baccoBright Yellow 2 (BY-2) suspension culture to understand the mechanisms of metal resistance in plant cells.We have analysed superoxide dismutase, catalase, and ascorbate peroxidase enzyme activities and superoxidedismutase-isoforms by isoelectric focusing gels in tobacco cells grown at two different toxic concentrations ofeach of the transition metals: copper, iron, manganese and zinc. Exposure of tobacco cells to these metals causedchanges in total superoxide dismutase activity in a different manner, depending on the metal assayed: after cop-perand manganese treatments, total superoxide dismutase activity was enhanced, while it was reduced after ironand zinc exposure. Superoxide dismutase-isoforms were affected by the metal used, and a Fe-SOD band with thesame isoelectric point as a Cu, Zn-SOD from non-treated cells, was induced after iron and zinc treatments. Cu,Zn-SODs were present in all metal-treatments whereas Mn-SOD was not detected in any case. Concerning otherantioxidant enzymes tested, such as catalase and ascorbate peroxidase, the latter showed a remarkable increase inactivity in response to copper treatments and catalase activity was enhanced after iron and with the lowest man-ganeseconcentration. Lipid peroxidation was increased after each metal treatment, as an indication of the oxi-dativedamage caused by metal concentration assayed in tobacco cells. These results suggest that an activation ofsome antioxidant enzymes in response to oxidative stress induced by transition metals is not enough to confertolerance to metal accumulation.

Ascorbate peroxidase Catalase Copper-zinc superoxide dismutase Iron-superoxide dismutase Manganese-superoxide dismutase Superoxide dismutase isoforms Tobacco cells Transition metals 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  1. 1.Dept. Bioquimica Biologia Molecular y Celular de PlantasEstacion Experimental Zaidin (C.S.I.C.)GranadaSpain
  2. 2.Dept. Nutrición y Fisiología VegetalCentro Edafología y Biología Aplicada del Segura (C.S.I.C.)MurciaSpain

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