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Plant Cell, Tissue and Organ Culture

, Volume 69, Issue 1, pp 79–83 | Cite as

In vitro regeneration of Echinacea purpurea from leaf explants

  • A. Koroch
  • H.R. Juliani
  • J. Kapteyn
  • J.E. SimonEmail author
Article

Abstract

Efficient plant regeneration was achieved via organogenesis from callus cultures derived from leaf tissue of Echinacea purpurea. Proliferating shoot cultures were obtained by placing leaf explants on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) combinations. MS medium supplemented with BAP (4.44 μM) and NAA (0.054 μM) was the most effective, providing high shoot regeneration frequencies (100%) associated with a high number of shoots per explant (7.7 shoots/explant). Plantlets were rooted on MS medium alone or in combination with different concentrations of indole-3-butyric acid (IBA), and high rooting and survival was achieved using MS media without plant growth regulators (PGR). All plantlets survived acclimatization producing healthy plants in the greenhouse. This study demonstrated that adventitious shoot regeneration of E. purpurea from leaf explants can be a useful method for the multiplication of this important medicinal plant.

organogenesis plant regeneration purple coneflower tissue culture 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • A. Koroch
    • 1
  • H.R. Juliani
    • 1
  • J. Kapteyn
    • 1
  • J.E. Simon
    • 1
    Email author
  1. 1.Center for New Use Agriculture and Natural Plant ProductsRutgers UniversityNew BrunswickUSA

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