In vitro regeneration of Echinacea purpurea from leaf explants
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Efficient plant regeneration was achieved via organogenesis from callus cultures derived from leaf tissue of Echinacea purpurea. Proliferating shoot cultures were obtained by placing leaf explants on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) combinations. MS medium supplemented with BAP (4.44 μM) and NAA (0.054 μM) was the most effective, providing high shoot regeneration frequencies (100%) associated with a high number of shoots per explant (7.7 shoots/explant). Plantlets were rooted on MS medium alone or in combination with different concentrations of indole-3-butyric acid (IBA), and high rooting and survival was achieved using MS media without plant growth regulators (PGR). All plantlets survived acclimatization producing healthy plants in the greenhouse. This study demonstrated that adventitious shoot regeneration of E. purpurea from leaf explants can be a useful method for the multiplication of this important medicinal plant.
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- Bauer R & Wagner H (1991) Echinacea species as potential immunostimulatory drugs. In: Wagner H & Farnsworth NR (eds) Economic and Medicinal Plant Research, Vol 5 (pp 253–321). Academic Press, LondonGoogle Scholar
- Choffe KL, Victor JMR, Murch SJ & Saxena PK (2000a) In vitro regeneration of Echinacea purpurea L. Direct somatic embryogenesis and indirect shoot organogenesis in petiole culture. In Vitro Cell Dev-Pl 36(1): 30–36Google Scholar
- Choffe KL, Murch SJ & Saxena PK (2000b) Regeneration of Echinacea purpurea: Induction of root organogenesis from hypocotyls and cotyledon explants. Plant Cell Tiss. Org. 62: 227–234Google Scholar
- Lisowska K & Wysokinska H (2000) In vitro propagation of Catalpa ovata G. Don. Plant Cell Tiss. Org. 60: 171–176Google Scholar
- McGregor RL (1968) The taxonomy of the Genus Echinacea (Compositae). The University of Kansas Science Bulletin. Vol XLVIII: 113–142Google Scholar
- McKeown KA (1999) A Review of taxonomy of the Genus Echinacea. In: Janick J (ed) Perspectives on New Crops and New Uses (pp 482–490). Purdue University. USAGoogle Scholar
- Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
- Pereira AM, Bertoni BW, Appezzato-da-Glória B, Araujo ARB, Januário AH, Lourenco MV & Franca SC (2000) Micropropagation of Pothomorphe umbellate via direct organogenesis from leaf explants. Plant Cell Tiss. Org. 60: 47–53Google Scholar
- Pretto FR & Santarém ER (2000) Callus formation and regeneration from Hypericum perforatum leaves. Plant Cell Tiss. Org. 62: 107–113Google Scholar
- Schwarz OJ & Beaty RM (2000) Organogenesis. In: Trigiano RN & Gray DJ (eds) Plant Tissue Culture Concept and Laboratories Exercises. CRC Press, Washington, DCGoogle Scholar
- Skoog F & Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues culture in vitro. Symp. Soc. Exp. Biol. 11: 118–131Google Scholar
- Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh PC & Zimmerman RH (eds) Micropropagation. Kluwer Academic Publishers, Dordrecht, The NetherlandsGoogle Scholar