Chromosome Research

, Volume 10, Issue 2, pp 109–116 | Cite as

Complex structure of B-chromosomes in two mammalian species: Apodemus peninsulae (Rodentia) and Nyctereutes procyonoides (Carnivora)

  • V. A. Trifonov
  • P. L. Perelman
  • S.-I. Kawada
  • M. A. Iwasa
  • S.-I. Oda
  • A. S. Graphodatsky

Abstract

B-chromosomes (Bs) of two mammalian species, raccoon dog (Nyctereutes procyonoides, Carnivora) and Asian wood mouse (Apodemus peninsulae, Rodentia) were investigated using chromosome segment microdissection and double-colour FISH. In the raccoon dog, all B-chromosomes showed homology with each other but not with the A-chromosomes. Two segment-specific probes (from proximal and distal parts of B) have been localized in corresponding chromosome parts, with significant variation in their sizes. In Asian wood mice, two types of B-specific chromatin were revealed – B1 and B2. Most Bs were either B1 or B2 specific; furthermore, some Bs were found to be composed of both types of chromatin. B-chromosome-specific libraries of A. peninsulae contain sequences homologous to the heterochromatic regions of sex and some A-chromosomes and dispersed repeated sequences. B1-specific probes gave signals on sex chromosomes of Apodemus speciosus and Apodemus agrarius. The origin and evolution of B-chromosomes in mammals are discussed.

Apodemus B-chromosome cytogenetics microdissection Nyctereutes raccoon dog wood mice 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • V. A. Trifonov
    • 1
  • P. L. Perelman
    • 1
  • S.-I. Kawada
    • 2
  • M. A. Iwasa
    • 3
  • S.-I. Oda
    • 2
  • A. S. Graphodatsky
    • 1
  1. 1.The Institute of Cytology and GeneticsNovosibirskRussia.
  2. 2.School of Agricultural SciencesNagoya UniversityNagoyaJapan
  3. 3.Graduate School of Environmental Earth ScienceHokkaido UniversitySapporoJapan

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