The Histochemical Journal

, Volume 33, Issue 9–10, pp 569–578 | Cite as

The Hypothalamo-hypophyseal System of the White Seabream Diplodus Sargus: Immunocytochemical Identification of Arginine-vasotocin, Isotocin, Melanin-concentrating Hormone and Corticotropin-releasing Factor

  • G. Duarte
  • M.M. Segura-Noguera
  • M.P. Martín del Río
  • J.M. Mancera
Article

Abstract

The distribution of the neurosecretory hormones vasotocin, isotocin and melanin-concentrating hormone and the hypophysio-tropic hormone corticotropin-releasing factor was studied in the hypothalamo-hypophyseal system of the white seabream (Diplodus sargus) using immunocytochemical techniques. Magnocellular and parvocellular perikarya immunoreactive for arginine-vasotocin and isotocin were present in the nucleus preopticus. Perikarya immunoreactive for arginine-vasotocin extended more caudally with respect to isotocin-immunoreactive perikarya. Parvocellular perikarya were located at rostroventral levels and magnocellular perikarya in the dorsocaudal portion of the nucleus. Arginine-vasotocin and isotocin did not coexist in the same neuron. Fibres immunoreactive for arginine-vasotocin and isotocin innervated all areas of neurohypophysis and terminate close to corticotropic and melanotropic cells. Perikarya immunoreactive for melanin-concentrating hormone and corticotropin-releasing factor were observed in the nucleus lateralis tuberis, with a few neurons in the nucleus periventricularis posterior. In addition, melanin-concentrating hormone immunoreactive perikarya were detected in the nucleus recessus lateralis. The preoptic nucleus did not show immunoreactivity for these antisera. Fibres showing melanin-concentrating hormone and corticotropin-releasing factor immunoreactivity ended close to the melanotropic and somatolactotrophic cells of the pars intermedia, and close to the corticotrophic cells of the rostral pars distalis.

Keywords

Corticotrophic Cell Vasotocin Immunoreactive Perikaryon Immunocytochemical Identification Factor Immunoreactivity 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • G. Duarte
    • 1
    • 2
  • M.M. Segura-Noguera
    • 1
  • M.P. Martín del Río
    • 1
  • J.M. Mancera
    • 3
  1. 1.Departamento de Biología Animal, Facultad de Ciencias del MarUniversidad de Cádiz, Puerto RealCádizSpain
  2. 2.Departamento de Zoología e Genética, Instituto de BiologíaUniversidade Federal de PelotasBrasil
  3. 3.Departamento de Biología Animal, Facultad de Ciencias del MarUniversidad de Cádiz, Puerto RealCádizSpain

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