Molecular and Cellular Biochemistry

, Volume 232, Issue 1–2, pp 19–26 | Cite as

Early changes in myocardial antioxidant enzymes in rats treated with adriamycin

  • Timao Li
  • Igor Danelisen
  • Pawan K. Singal
Article

Abstract

Increased oxidative stress and antioxidant deficit have been suggested to play a major role in adriamycin-induced cardiomyopathy and congestive heart failure due to multiple treatments with adriamycin (doxorubicin). In this study, we investigated the acute effects of a single dose of adriamycin on myocardial antioxidant enzymes in rats. Adriamycin (2.5 mg/kg) was injected (i.p.) and myocardial antioxidant enzyme activities, mRNA abundance and protein levels at 1, 2, 4 and 24 h were examined. While manganese superoxide dismutase (MnSOD), glutathione peroxidase (GSHPx) and catalase (CAT) activities were not significantly changed, copper-zinc superoxide dismutase (CuZnSOD) activity was reduced at all time points and this change correlated with a decrease in its protein content. CuZnSOD mRNA was increased at 1 and 24 h. GSHPx mRNA and protein levels were transiently decreased by 20 and 25% respectively at 2 h. MnSOD mRNA was not significantly changed, but its protein levels were significantly decreased at 1 h. Lipid peroxidation was increased transiently at 1, 2 and 4 h. A transient depression in antioxidant enzyme as well as transient increase in oxidative stress with a single dose of adriamycin may precede more sustained changes seen with the repeated administration of the drug and contribute to the development of cardiomyopathy and heart failure.

antioxidants lipid peroxidation oxidative stress doxorubicin cardiomyopathy heart failure 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Timao Li
  • Igor Danelisen
  • Pawan K. Singal

There are no affiliations available

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