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Glycoconjugate Journal

, Volume 18, Issue 5, pp 391–400 | Cite as

Characterization of the sugar-binding specificity of the toxic lectins isolated from Abrus pulchellus seeds

  • Marcio V. Ramos
  • Alexandre H. Sampaio
  • Benildo S. Cavada
  • Juan J. Calvete
  • Thalles B. Grangeiro
  • Henri Debray
Article

Abstract

The sugar-binding specificity of the toxic lectins from Abrus pulchellus seeds was investigated by combination of affinity chromatography of glycopeptides and oligosaccharides of well-defined structures on a lectin-Sepharose column and measurement of the kinetic interactions in real time towards immobilized glycoproteins. The lectins showed strong affinity for a series of bi- and triantennary N-acetyllactosamine type glycans. The related asialo-oligosaccharides interact more strongly with the lectins. The best recognized structures were asialo-glycopeptides from fetuin. Accordingly, the kinetic interaction with immobilized asialofetuin was by far the most pronounced. Human and bovine lactotransferrins and human serotransferrin interacted to a lesser extent. The interaction with asialofetuin was inhibited by galactose in a dose dependent manner. Lactose, N-acetyllactosamine and lacto-N-biose exhibited similar degree of inhibition while N-acetylgalactosamine was a poor inhibitor. These results suggested that the carbohydrate-binding site of the Abrus pulchellus lectins was specific for galactose and possess a remarkable affinity for the sequences lactose [β-D-Gal-(1→4)-D-Glc], N-acetyllactosamine [β-D-Gal-(1→4)-D-GlcNAc] and lacto-N-biose [β-D-Gal-(1→3)-D-GlcNAc].

Abrus pulchellus lectin plant toxin protein-carbohydrate interaction surface plasmon resonance 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Marcio V. Ramos
    • 1
  • Alexandre H. Sampaio
    • 2
  • Benildo S. Cavada
    • 3
  • Juan J. Calvete
    • 4
  • Thalles B. Grangeiro
    • 5
  • Henri Debray
    • 6
  1. 1.Departamentos de BiologiaUniversidade Federal do Ceara, Campus do PiciFortaleza-CearaBrasil
  2. 2.Engenharia de pescaUniversidade Federal do Ceara, Campus do PiciFortaleza-CearaBrasil
  3. 3.Bioquimica e Biologia MolecularUniversidade Federal do Ceara, Campus do PiciFortaleza-CearaBrasil
  4. 4.Intituto de Biomedicina de ValenciaC.S.I.C.ValenciaSpain
  5. 5.Departamentos de BiologiaBrasil
  6. 6.Laboratoire de Biochimie et UMR N.8576 du CNRSUniversité des Sciences et Technologies de LilleVilleneuve D'Ascq CEDEXFrance

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