Advertisement

Plant Growth Regulation

, Volume 36, Issue 1, pp 87–90 | Cite as

Effects of phytosulfokine-α on growth and tropane alkaloid production in transformed roots of Atropa belladonna

  • Kazuo SasakiEmail author
  • Tatsuaki Ishise
  • Koichiro Shimomura
  • Toshihiro Kobayashi
  • Yoshikatsuo Matsubayashi
  • Youji Sakagami
  • Hironori Umetsu
  • Hiroshi Kamada
Article

Abstract

Phytosulfokine (PSK)-α is a sulphated pentapeptide, isolated fromthe medium of cultured Asparagus officinalis mesophyllcells, that promotes cell proliferation. It is a putative key factor inconditioned medium required for the growth of low-density plant cell cultures.The present study investigates the effect of PSK-α on growth and tropanealkaloid production in Atropa belladonna hairy rootstransformed with Agrobacteriumrhizogenes (MAFF 03-01724). Although the growth rates ofhairy roots cultured in medium with orwithout PSK-α for 4 weeks did not show any differences, the productivityof tropane alkaloids, especially of hyoscyamine, was enhanced by10−7 or 10−8 M PSK-α. Inaddition, the content of tropane alkaloids in transformed roots treated withPSK-α was 1.4 times higher than that of untreated roots after 4 weeks ofculture. The time course of growth and tropane alkaloid production inAtropa belladonna transformed roots suggested thatPSK-α influenced the growth of transformed roots during the activegrowingphase, but not tropane alkaloid production.

Atropa belladonna Hairy roots Tropane alkaloid 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Hanai H., Matsuno T., Yamamoto M., Matsubayashi Y., Kobayashi T., Kamada H. et al. 2000. A secreted peptide growth factor, phytosulfokine, acting as a stimulatory factor of carrot somatic embryo formation. Plant Cell Physiol. 41: 27–32.PubMedGoogle Scholar
  2. Kobayashi T., Eun C.H., Hanai H., Matsubayashi Y., Sakagami Y. and Kamada H. 1999. Phytosulfokine-α, a peptidyl plant growth factor, stimulates somatic embryogenesis in carrot. J. Exp. Bot. 50: 1123–1128.Google Scholar
  3. Matsubayashi Y., Hanai H., Hara O. and Sakagami Y. 1996. Active fragments and analogues of the plant growth factor, phytosulfokine: structure-activity relationships. Biochem. Biophys. Res. Commun. 225: 209–214.PubMedGoogle Scholar
  4. Matsubayashi Y. and Sakagami Y. 1996. Phytosulfokine, sulfated peptides that induce the proliferation of single mesophyll cells of Asparagus officinalis L. Proc. Natl. Acad. Sci. USA. 93: 7623–7627.PubMedGoogle Scholar
  5. Matsubayashi Y., Takagi L. and Sakagami Y. 1997. Phytosulfokine-α, sulfated pentapeptide, stimulates the proliferation of rice cells by means of specific high-and low-affinity binding sites. Proc. Natl. Acad. Sci. USA 94: 13357–13362.PubMedGoogle Scholar
  6. Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 73–497.Google Scholar
  7. Nakanishi F., Sasaki K. and Shimomura K. 1998. Isolation and identification of littorine from hairy roots of Atropa belladonna. Plant Cell Rep. 18: 249–251.Google Scholar
  8. Yamakawa S., Matsubayashi Y., Sakagami, Y., Kamada H. and Satoh S. 1999. Promotive effects of the peptidyl plant growth factor, Phytosulfokine-α, on the growths and chlorophyll content of Arabidopsis seedlings under high night-time temperature conditions. Biosci. Biotechnol. Biochem. 63: 2240–2243.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Kazuo Sasaki
    • 1
    Email author
  • Tatsuaki Ishise
    • 1
  • Koichiro Shimomura
    • 2
  • Toshihiro Kobayashi
    • 3
  • Yoshikatsuo Matsubayashi
    • 4
  • Youji Sakagami
    • 4
  • Hironori Umetsu
    • 1
  • Hiroshi Kamada
    • 3
  1. 1.Faculty of EngineeringAomori UniversityAomoriJapan
  2. 2.Tsukuba Medicinal Plant Research StationNational Institute of Health SciencesTsukubaJapan
  3. 3.Institute of Biological SciencesUniversity of TsukubaTsukubaJapan
  4. 4.School of Agricultural ScienceNagoya UniversityChikusa, NagoyaJapan

Personalised recommendations