Effects of phytosulfokine-α on growth and tropane alkaloid production in transformed roots of Atropa belladonna
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Phytosulfokine (PSK)-α is a sulphated pentapeptide, isolated fromthe medium of cultured Asparagus officinalis mesophyllcells, that promotes cell proliferation. It is a putative key factor inconditioned medium required for the growth of low-density plant cell cultures.The present study investigates the effect of PSK-α on growth and tropanealkaloid production in Atropa belladonna hairy rootstransformed with Agrobacteriumrhizogenes (MAFF 03-01724). Although the growth rates ofhairy roots cultured in medium with orwithout PSK-α for 4 weeks did not show any differences, the productivityof tropane alkaloids, especially of hyoscyamine, was enhanced by10−7 or 10−8 M PSK-α. Inaddition, the content of tropane alkaloids in transformed roots treated withPSK-α was 1.4 times higher than that of untreated roots after 4 weeks ofculture. The time course of growth and tropane alkaloid production inAtropa belladonna transformed roots suggested thatPSK-α influenced the growth of transformed roots during the activegrowingphase, but not tropane alkaloid production.
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