Advertisement

Plant Growth Regulation

, Volume 36, Issue 1, pp 81–85 | Cite as

Thidiazuron-induced shoot-bud formation on root segments of Albizzia julibrissin is an apex-controlled, light-independent and calcium-mediated response

  • M. Hosseini-Nasr
  • A. Rashid
Article

Abstract

Root segments or entire roots of Albizziajulibrissin formed shoot-buds; the former were more responsive thanthe latter. The regeneration capacity of root segments increased with anincreasing distance from the meristem. Shoot regeneration on N6mineral formulation required either a cytokinin (BAP) or thidiazuron (TDZ); thelatter was more effective than the former, inducing a higher number of shoots ata low concentration (0.1 μM) in the light as well as in thedark. The frequency of shoot formation was reduced when the auxin inhibitorsmaleic hydrazide (MH) or triiodobenzoic acid (TIBA) were included, indicating anindirect role of auxin in shoot morphogenesis. Inhibitors of calcium uptake(lanthanum) and calmodulin, trifluoperazine (TFP) or chlorpromazine (CPZ) atvery low levels, resulted in inhibition to reduction in frequency of shootmorphogenesis. This indicates that TDZ-induced shoot formation may be acalcium-mediated response. Increasing the level of calcium in the medium did notpromote shoot formation in the presence of TDZ (0.1 μM). At areduced level of calcium, which was ineffective in the presence of low TDZ (0.1μM), shoot-buds appeared when the concentration of TDZ wasraised to 1.0 μM. This provides indirect evidence that TDZmodulates the tissue level of calcium needed for shoot formation.

Albizzia Calcium Root segment Shoot Thidiazuron 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ahee J. and Duhoux E. 1994. Root culturing of Faiderbia - Acacia albida as a source of explants for shoot regeneration. Plant Cell Tissue Organ. Cult. 36: 219–225.Google Scholar
  2. Akashi R., Hoffman-Tsy S.S. and Hoffman F. 1998. Selection of a super growing legume root culture that permits controlled switching between root cloning and direct embryogenesis. Theor. Appl. Genet. 96: 758–764.Google Scholar
  3. Badzian T. and Rybczynski J.J. 1994. Cytokinin control of shoot regeneration in root segment culture of Lotus corniculatus seedlings. Acta Physiol. Plant 16: 61–67.Google Scholar
  4. Butcher D. and Street.E. 1964. Excised root culture. Biol. Rev. 30: 513.Google Scholar
  5. Capelle S.C., Mok D.W.S., Kirchner S.C. and Mok M.C. 1983. Effects of thidiazuron on cytokinin autonomy and the metabolism of N6-(Y2-isopentenyl) [8-14C] adenosine in callus tissues of Phaseolus lunatus L. Plant Physiol. 73: 796–802.Google Scholar
  6. Chu C.C. 1978. The N6 medium and its applications to anther of cereal crops. In: Proc Symp on plant tissue culture. Science Press, Beijing, China, pp. 43–50.Google Scholar
  7. Croxton F.E. and Cowden D.J. 1966. Applied General Statistics. Prentice Hall of India, New Delhi.Google Scholar
  8. Elliott D.C. 1983. Inhibition of cytokinin-regulated responses by calmodulin-binding compounds. Plant Physiol. 72: 215–218.Google Scholar
  9. Espinoza N.D. and Dodds J.H. 1985. Adventitious shoot formation on cultured potato root. Plant Sci. 41: 121–124.Google Scholar
  10. Gill R. and Saxena P.K. 1992. Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron. Can. J. Bot. 70: 1186–1192.Google Scholar
  11. Huetteman C.A. and Preece J.E. 1993. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell. Tiss. Org. Cult. 33: 105–119.Google Scholar
  12. Hutchinson M.J., Krishnaraj S. and Saxena P.K. 1996. Morphological and physiological changes during thidiazuron-induced somatic embryogenesis in Geranium (Pelargonium x Hortorium bailey) hypocotyl cultures. Int. J. Pl. Sci. 157: 440–446.Google Scholar
  13. Iantcheva A., Vlahova M., Bakalova E., Kondorosi E., Elliott M.C. and Atanassov A. 1999. Regeneration of diploid annual medics via direct somatic embryogenesis promoted by thidiazuron and benzylaminopurine. Plant Cell. Rep. 18: 904–910.Google Scholar
  14. Laloue M. and Fox J.E. 1989. Cytokinin oxidase from wheat. Plant Physiol. 90: 899–906.Google Scholar
  15. Li Z.l. and Wang S.Y. 1988. Reduction of abscisic acid content and induction of sprouting in potato, Solanum tuberosum L. by TDZ. J. Plant Growth Regul. 7: 37–44.Google Scholar
  16. Chin-Yi 1993. The use of Thidiazuron in tissue culture. In vitro Cell. Dev. Biol. 29: 92–96.Google Scholar
  17. Malik K.A. and Saxena P.K. 1992. Regeneration in Phaseolus vulgaris L. high frequency induction of direct shoot formation on intact seedlings by N6-benzylaminopurine and TDZ. Planta 186: 384–389.Google Scholar
  18. Malik K.A. and Saxena P.K. 1992a. Somatic embryogenesis and shoot regeneration from intact-seedlings of Phaseolus aconitifolius, P. aureus, P. coccineus and P. wrightii. Plant Cell. Rep. 11: 163–168.Google Scholar
  19. Mok M.C., Mok D.W.S. and Armstrong D.J. 1982. Cytokinin activity of N-phenyl-N′-12,3,thidiazol-5-yl urea (Thidiazuron). Phytochemistry 21: 1509–1511.CrossRefGoogle Scholar
  20. Mok M.C., Mok D.W.S., Turner J.E. and Mujer C.V. 1987. Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems. Hort. Sci. 22: 1194–1197.Google Scholar
  21. Murthy B.N.S., Murch S.J. and Saxena P.K. 1995. TDZ-induced somatic embryogenesis in intact seedlings of peanut (Arachis hypogaea): endogenous growth regulator levels and signifi cance of cotyledons. Physiol. Plant 94: 268–276.Google Scholar
  22. Nef-Campa C., Chaintreuil-Dongmo C. and Dreyfus B.L. 1996. Regeneration of the tropical legume Aeschynomene sensitiva SW. from root explants. Plant Cell. Tissue Organ. Cult. 44: 149–154.Google Scholar
  23. Parker R.E. 1979. Introductory Statistics for Biology. Edward-Arnold, London.Google Scholar
  24. Pearson E.S. and Hartley H.O. 1962. Biometrika, Tables for Statisticians. Cambridge University Press.Google Scholar
  25. Preece J.E., Huetteman C.A. and Ashley W.C. 1988. Provenance tests for biomass production using micropropagated clonal silver maple. Hort. Sci. 23: 803.Google Scholar
  26. Roberts D.M. and Harmon A.C. 1992. Calcium-modulated proteins: Targets of intracellular calcium signals in higher plants. Ann. Rev. Plant Physiol Plant Mol. Biol. 43: 375–414.Google Scholar
  27. Sankhla D., Davis T.D. and Sankhla N. 1994. Thidiazuron-induced in vitro shoot formation from roots of intact seedlings of Albizzia julibrissin. Plant Growth Reg. 14: 267–272.Google Scholar
  28. Saxena P.K., Malik K.A. and Gill R. 1992. Induction by Thidiazuron of somatic embryogenesis in intact seedlings of peanut. Planta 187: 421–424.CrossRefGoogle Scholar
  29. Singha S. and Bhatia S.K. 1988. Shoot proliferation of pear cultivars on medium containing thidiazuron and benzylaminopurine. Hort. Sci. 23: 803.Google Scholar
  30. Thomas J.C. and Katterman F.R. 1986. Cytokinin activity induced by thidiazuron. Plant Physiol. 81: 681–683.Google Scholar
  31. Trewavas A. 1999. Calcium makes waves. Plant Physiol. 120: 1–6.PubMedGoogle Scholar
  32. Van Niewwkerk J.P., Zimmerman R.H. and Fordham I. 1986. Thidiazuron stimulation of apple shoot proliferation in vitro. Hort. Sci. 21: 516–518.Google Scholar
  33. Visser C., Qureshi J.A., Gill R. and Saxena P.K. 1992. Morphoregulatory role of thidiazuron-substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in Geranium hypocotyl cultures. Plant Physiol. 99: 1704–1707.Google Scholar
  34. Wayne R. and Hepler P.K. 1984. The role of calcium ions in phytochrome-mediated germination of spores of Onoclea sensiblis. Planta 160: 12–20.Google Scholar
  35. Yip W.K. and Yang S.F. 1986. Effect of thidiazuron, in cytokinindependent ethylene production systems. Plant Physiol. 80: 515–519.Google Scholar
  36. White P.R. 1934. Potentially unlimited growth of excised roots. Plant Physiol. 9: 585–600.Google Scholar
  37. Zelcher A., Soferman O. and Izhar S. 1983. Shoot regeneration in root cultures of Solanaceae. Plant Cell. Rep. 2: 252–254.Google Scholar
  38. Zhao Z. and Ross C.W. 1989. Effects of compounds that influence calcium absorption and of calcium-calmodulin inhibitors on zeatin-induced growth and chlorophyll synthesis in excised cucumber cotyledons. Plant Cell. Physiol. 30: 793–800.Google Scholar
  39. Zimmerman T.W. and Scorza R. 1992. Shoot growth and proliferation of peach under varying environmental regimes. Hort. Sci. 27: 696.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • M. Hosseini-Nasr
    • 1
  • A. Rashid
    • 1
  1. 1.Department of BotanyUniversity of DelhiDelhiIndia

Personalised recommendations