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Biotechnology Letters

, Volume 24, Issue 5, pp 335–341 | Cite as

Characterisation of anaerobic mixed cultures by partition in an aqueous two-phase system

  • Marika Murto
  • Ulla Dissing
  • Bo Mattiasson
Article

Abstract

A method to characterise a dynamic microbial consortium is described. By exploiting differences in surface properties between different cells and between cells of different physiological status, it was possible to develop a partition pattern for a mixed culture under different conditions. The separation method used was partition in aqueous two-phase systems and when using a counter current extraction process one could clearly differentiate the partition profile between resting, active and overloaded biomethanation cultures.

anaerobic degradation aqueous two-phase systems counter-current distribution mixed culture partitioning of cells 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Marika Murto
    • 1
  • Ulla Dissing
    • 1
  • Bo Mattiasson
    • 1
  1. 1.Department of BiotechnologyLund UniversityLundSweden

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