Virus Genes

, Volume 24, Issue 2, pp 99–105 | Cite as

Characterization of the Glycoprotein B Gene from Ruminant Alphaherpesviruses

  • Carlos Ros
  • Sándor Belák


The complete open reading frame and promoter region of the glycoprotein B (gB) gene has been identified and sequenced from five poorly characterized alphaherpesviruses of ruminants, bovine herpesvirus 5 (BHV-5), buffalo herpesvirus 1 (BuHV-1), cervine herpesvirus 1 (CerHV-1), rangiferine herpesvirus 1 (RanHV-1), and caprine herpesvirus 1 (CapHV-1). One of the two regions identified with considerable sequence and length variation is also target of the immune system, as two B cell epitopes have been identified in this location [33]. Features shared with bovine herpesvirus 1 (BHV-1) gB include two broad hydrophobic regions, six N-glycosylation sites and ten conserved cysteine residues in the gB extracellular domain. Phylogenetic analysis showed that the studied ruminant alphaherpesviruses form, together with BHV-1, a consistent group within the α2 subgroup of the herpesviruses. BHV-5 and BuHV-1 are most closely related to BHV-1, followed by CerHV-1, RanHV-1 and more distantly by CapHV-1. A remarkable high degree of sequence similarity was observed between BuHV-1 and the neuropathogenic BHV-5.

BHV-1 glycoprotein B phylogeny ruminant alphaherpesvirus 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Carlos Ros
    • 1
    • 2
  • Sándor Belák
    • 3
  1. 1.Department of Chemistry and BiochemistryUniversity of BernBern
  2. 2.ZLB Bioplasma AGBernSwitzerland
  3. 3.Department of VirologyThe National Veterinary Institute, Biomedical CenterUppsalaSweden

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