Molecular and Cellular Biochemistry

, Volume 231, Issue 1–2, pp 69–74 | Cite as

Identification of TH1 as an interaction partner of A-Raf kinase

  • Xiang L. Yin
  • She. Chen
  • Jian X. Gu


A-Raf is an important intermediate of the growth factor Ras-MAP kinase pathway. In a two-hybrid screen of human fetal liver cDNA library, TH1 was detected as a new interaction partner of A-Raf. TH1 is a highly conserved and widely expressed protein, which was recently cloned by Bonthron DT group. The binding between A-Raf and TH1 was specific, as no binding between TH1 and B-Raf or c-Raf was observed, and the amino-terminal 162 amino acids in the A-Raf regulatory domain were found to be sufficient for this interaction. This specific interaction may have played a critical role in the activation of A-Raf.

A-Raf kinase interaction putative TH1 protein two-hybrid system 


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© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Xiang L. Yin
  • She. Chen
  • Jian X. Gu

There are no affiliations available

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