Advertisement

Journal of Materials Science: Materials in Medicine

, Volume 12, Issue 9, pp 807–810 | Cite as

Immobilization of the thrombin inhibitor r-hirudin conserving its biological activity

  • Jörg Lahann
  • Wilhelm Plüster
  • Doris Klee
  • Hans-Gregor Gattner
  • Hartwig Höcker
Article

Abstract

Surface immobilization of the thrombin inhibitor r-hirudin was carried out on two different polymers. Linkage to poly(urethane-graft-acrylic acid) (PAC/PU) was done via carboxylic acid groups, using a water soluble carbodimide, while the immobilization on a modified poly[(ethene-co-vinyl acetate)-graft-vinyl chloride] (PVC/EVA) was achieved via the alcohol groups of the polymer using HDI as spacer. Direct immobilization of r-hirudin leaded to a remarkable loss of thrombin activity. As proved by means of protein chemical analysis, loss of activity was due to a selective coupling via the N-terminal amino group of r-hirudin, which is essential for its thrombin activity. Based on these results we developed an immobilization method via an ε-amino group of r-hirudin preserving full biological activity of the r-hirudin coated surface. © 2001 Kluwer Academic Publishers

Keywords

Polymer Alcohol Biological Activity Immobilization Carboxylic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    G. Agnelli, Cardiovascular Research 31 (1996) 232.Google Scholar
  2. 2.
    S. M. Seiler, Seminars in Thrombosis and Haemostasis 22 (1996) 223.Google Scholar
  3. 3.
    S. W. Kim and H. Jacobs, Blood Purification 4 (1996) 357.Google Scholar
  4. 4.
    T. E. Warkentin, Drug Safety 17 (1997) 325.Google Scholar
  5. 5.
    J. Dodt, Angewandte Chemie 107 (1995) 867.Google Scholar
  6. 6.
    T. J. Rydel, K. G. Ravichandran, A. Tulinsky, W. Bode, R. Huber, C. Roitsch and J. W. Fenton II, Science 249 (1990) 277.Google Scholar
  7. 7.
    J.-Y. Chang, FEBS Letters 164 (1983) 307.Google Scholar
  8. 8.
    J.-C. Sheehan, P. A. Cuickshank and G. I. Boshart, Journal of Organic Chemistry 26 (1961) 2525.Google Scholar
  9. 9.
    G. J. Teller and J. C. Balreth-Geers, International Journal of Peptide and Protein Research 7 (1975) 295.Google Scholar
  10. 10.
    A. Tulinsky, Seminars in Thrombosis and Haemostasis 22 (1996) 117.Google Scholar
  11. 11.
    J. Lahann, D. Klee, W. PlÜster and H. HÖcker, Biomaterials 22 (2001) 817.Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Jörg Lahann
    • 1
  • Wilhelm Plüster
    • 1
  • Doris Klee
    • 1
  • Hans-Gregor Gattner
    • 1
  • Hartwig Höcker
    • 1
  1. 1.Department of Macromolecular and Textile ChemistryRWTH AachenAachenGermany

Personalised recommendations