Journal of Materials Science: Materials in Medicine

, Volume 13, Issue 2, pp 225–232

Osteogenic proteins (bone sialoprotein and bone morphogenetic protein-7) and dental pulp mineralization

  • N. Six
  • F. Decup
  • J.-J. Lasfargues
  • E. Salih
  • M. Goldberg


Bone sialoprotein (BSP) cross-linked to collagen/gelatin was implanted in the pulp of rat’s upper molars. Comparison was carried out with a sham group (non implanted), with a group of rats receiving the carrier alone, and a group of molars where the perforated pulps were capped with calcium hydroxide. The cavities were occluded with a glass-ionomer cement (GIC). After 8, 14 and 30 days respectively the rats were killed by intracardiac perfusion of the fixative and processed for light microscopy. Dentin and predentin debris pushed into the pulp during the preparation enhanced self-repair processes, with large pulp remnants. The carrier alone induced slight inflammation, and calcium hydroxide the formation of a reparative dentin bridge. BSP stimulated the recruitment of cells which produced an homogeneous atubular dentin-like structure, filling after one month the mesial third of the crown pulp. Osteogenic protein (OP-1) used in the same experimental conditions induced the formation of osteodentin in the coronal pulp and the radicular part of the pulp was totally filled by a mineralized material. The differences reported here suggest two possible different therapeutic approaches with the two osteogenic proteins, BSP inducing pulp mineralization in the crown part, and OP-1 occluding the root part of the pulp.


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  1. 1.
    I. A. MjÖr, E. Dahl and C. F. Cox, J. Oral Pathol. Med. 20 (1991) 496.Google Scholar
  2. 2.
    U. SchrÖder, J. Dent. Res. 64 (1985) 541.Google Scholar
  3. 3.
    M. R. Urist, Science 150 (1965) 893.Google Scholar
  4. 4.
    A. H. Reddi, Nature Biotech. 16 (1998) 247.Google Scholar
  5. 5.
    V. Rosen and R. S. Thies, Trends Genet. 8 (1992) 97.Google Scholar
  6. 6.
    R. B. Rutherford, J. Wahle, M. Tucker, D. Rueger and M. Charette, Arch. Oral Biol. 38 (1993) 571.Google Scholar
  7. 7.
    R. B. Rutherford, L. Spangberg, M. Tucker, D. Rueger and M. Charette, ibid. 39 (1994) 833.Google Scholar
  8. 8.
    M. Nakashima, ibid. 35 (1990) 493.Google Scholar
  9. 9.
    M. Nakashima, ibid. 39 (1994) 1085.Google Scholar
  10. 10.
    Y. Lianjia, G. Yuhao and F. H. White, Clin. Orthop. 295 (1993) 305.Google Scholar
  11. 11.
    T. Takata, J. A. D'errico, K. B. Atkins, J. E. Berry, C. Strayhorn, R. S. Taichman and M. J. Somerman J. Periodontol. 69 (1998) 1247.Google Scholar
  12. 12.
    D. Tziafas, I. Kolokuris, A. Alvanou and K. Kaidoglou, Arch. Oral Biol. 37 (1992) 119.Google Scholar
  13. 13.
    D. Tziafas, N. Panagiotakopoulos and A. Kommenou, ibid. 40 (1995) 23.Google Scholar
  14. 14.
    A. J. Smith, R. S. Tobias, N. Cassidy, C. G. Plant, R. M. Browne, C. Beguekirn, J. V. Ruch and H. Lesot, ibid. 39 (1994) 13.Google Scholar
  15. 15.
    F. Decup, N. Six, B. Palmier, D. Buch, J.-J. Lasfargues, E. Salih and M. Goldberg, Clinical Oral Invest. (2000) (in press).Google Scholar
  16. 16.
    J. Chen, H. S. Shapiro and J. Sodek, J. Bone Miner. Res. 7 (1992) 987.Google Scholar
  17. 17.
    J. Chen, C. A. G. Mcculloh and J. Sodek, Arch. Oral Biol. 38 (1993) 241.Google Scholar
  18. 18.
    A. Franzen and D. Heinegard, Biochem. J. 232 (1985) 715.Google Scholar
  19. 19.
    K. Takeda, S. Oida, M. Goseki, T. Iimura, Y. Maruoka, T. Amagasa and S. Sasaki, Bone 15 (1994) 467.Google Scholar
  20. 20.
    T. Toyono, M. Nakashima, S. Kuhara and A. Akamine, J. Dent. Res. 76 (1997) 1555.Google Scholar
  21. 21.
    G. K. Hunter and H. A. Goldberg, Proc. Natl. Acad. Sci. USA 90 (1994) 8562.Google Scholar
  22. 22.
    G. K. Hunter and H. A. Goldberg, Biochem. J. 302 (1994) 175.Google Scholar
  23. 23.
    A. Oldberg, A. Franzen and D. Heinegard, J. Biol. Chem. 263 (1988), 19430.Google Scholar
  24. 24.
    R. Fujisawa and Y. Kuboki, Calcif. Tissue Int. 51 (1992) 438.Google Scholar
  25. 25.
    J. Wang, M. J. Glimcher, J. Mah, H.-Y. Zhou and E. Salih, Bone 22 (1998) 621.Google Scholar
  26. 26.
    J. Wang, J. G. Kennedy, J. R. Kasser, M. J. Glimcher and E. Salih, Orthopaed. Transact. 22 (1994) 951.Google Scholar
  27. 27.
    J. Wang and M. J. Glimcher, Calcif. Tissue Int. 65 (1999) 486.Google Scholar
  28. 28.
    Idem., ibid. 65 (1999) 156.Google Scholar
  29. 29.
    T. K. Sampath, J. C. Maliakal, P. V. Hauschka, W. K. Jones, H. Sasak, R. F. Tucker, K. H. White, J. E. Coughlin, M. M. Tucker and R. H. Pang, J. Biol. Chem. 267 (1992) 20352.Google Scholar
  30. 30.
    E. Salih, H.-Y. Zhou and M. J. Glimcher, ibid. 271 (1996) 16897.Google Scholar
  31. 31.
    M. Fitzgerald, D. J. Chiego and D. R. Heys, Arch. Oral Biol. 9 (1990) 707.Google Scholar
  32. 32.
    H. Lesot, C. BÈgue-Kirn, M. D. Kubler, J. M. Meyer, A. J. Smith, N. Cassidy and J. V. Ruch, Cells Materials 3 (1993) 201.Google Scholar
  33. 33.
    L. Stanislawski, J. P. Carreau, M. Pouchelet, Z. H. J. Chen and M. Goldberg, Clin. Oral Invest. 1 (1997) 131.Google Scholar
  34. 34.
    H. Y. Zhou, H. Takita, R. Fujisawa, M. Mizumo and Y. Kuboki, Calcif. Tissue Int. 56 (1995) 403.Google Scholar
  35. 35.
    D. Kobayashi, H. Takita, M. Mizuno, Y. Totsuka and Y. Kuboki, J. Biochem. 119 (1996) 475.Google Scholar
  36. 36.
    H. C. Slavkin, C. Bessem, P. J. Bringas, M. Zeichner-David, A. Nanci and M. L. Snead, Differentiation 37 (1988) 26.Google Scholar
  37. 37.
    T. Inai, T. Kukita, Y. Ohsaki, K. Nagata, A. Kukita and K. Kurisu, Anat. Rec. 229 (1991) 259.Google Scholar
  38. 38.
    M. Nakamura, P. Jr. Bringas, A. Nanci, M. Zeichner-David, B. Ashdown and H. C. Slavkin, ibid. 238 (1994) 383.Google Scholar
  39. 39.
    T. Sawada and A. Nanci, Arch. Oral Biol. 40 (1995) 1029.Google Scholar
  40. 40.
    D. D. Bossshardt and A. Nanci, J. Bone Miner. Res. 12 (1997) 367.Google Scholar
  41. 41.
    N. Six, J.-J. Lasfargues and M. Goldberg, J. Dentisty (2000) (in press).Google Scholar
  42. 42.
    M. Goldberg, L. Stanislawski, E. Bonte, X. Daniau and J.-J. Lasfargues, in “Advances in Glass-ionomer cements”, edited by C. L. Davidson and I. A. MjÖr (Quintessence Publishing Co, Inc Chicago, 1999) p. 103.Google Scholar
  43. 43.
    A. H. R. Rowe, Brit. Dent. J. 122 (1967) 291.Google Scholar
  44. 44.
    G. W. Wade. Oral Surg. Oral Med. Oral Path. 28 (1969) 226.Google Scholar
  45. 45.
    D. F. Turner, C. F. Marfurt and C. Sattelberg, J. Dent. Res. 68 (1989) 1262.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • N. Six
    • 1
    • 2
  • F. Decup
    • 1
  • J.-J. Lasfargues
    • 1
  • E. Salih
    • 3
  • M. Goldberg
    • 1
  1. 1.Laboratoire de Biologie et Physiopathologie Crânio-FacialeFaculté de Chirurgie Dentaire UniversitéParis V
  2. 2.Department of Conservative DentistryPrince of Songkla UniversityThailand
  3. 3.Laboratory for the Study of Skeletal Disorders and Rehabilitation, Department of Orthopedic SurgeryHarvard Medical School and Children’s HospitalBostonUSA

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