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Biotechnology Letters

, Volume 24, Issue 1, pp 9–12 | Cite as

Production of mannitol by a novel strain of Candida magnoliae

  • Kyung-Hwa Song
  • Jung-Kul Lee
  • Ji-Yoon Song
  • Soon-Gyu Hong
  • Hong Baek
  • Sang-Yong Kim
  • Hyung-Hwan Hyun
Article

Abstract

A novel microorganism was isolated which is able to produce mannitol when grown in the presence of fructose and glucose as carbon sources. In flask culture in a medium containing 150 g fructose l−1, it yielded 67 g mannitol l−1 after 168 h. In fed-batch culture with 3–12% (w/v) fructose, production reached a maximum of 209 g mannitol l−1 after 200 h, corresponding to an 83% yield and a 1.03 g l−1 h−1 productivity. The isolated strain was identified as Candida magnoliae based on identical sequences in the D1/D2 domain of its 26S rDNA and a similar carbon source utilization pattern with C. magnoliae reference strains.

Candida magnoliae fed-batch fermentation sludge mannitol 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Kyung-Hwa Song
    • 1
  • Jung-Kul Lee
    • 2
  • Ji-Yoon Song
    • 2
  • Soon-Gyu Hong
    • 3
  • Hong Baek
    • 1
  • Sang-Yong Kim
    • 4
  • Hyung-Hwan Hyun
    • 1
  1. 1.Department of Bioscience and BiotechnologyHankuk University of Foreign StudiesMohyun, Yongin-si, Kyounggi-doKorea
  2. 2.BioNgene Co., Ltd.SeoulKorea
  3. 3.Korean Collection for Type CulturesKorea Research Institute of Bioscience and BiotechnologyYusong-ku, TaejonRepublic of Korea
  4. 4.Bolak. Co., Ltd.Hwasung-Kun, Kyungki-DoKorea

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