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Biotechnology Letters

, Volume 24, Issue 1, pp 27–33 | Cite as

A colorimetric method for the determination of lipase activity in soil

  • R. Margesin
  • G. Feller
  • M. Hämmerle
  • U. Stegner
  • F. Schinner
Article

Abstract

A colorimetric method for the determination of lipase activity in soil has been developed. Using p-nitrophenyl butyrate as substrate, soil samples are incubated at 30 °C and pH 7.25 for 10 min. After cooling on ice and centrifugation, the released p-nitrophenol is determined at 400 nm. To allow for the adsorption of p-nitrophenol onto soil, a calibration curve is prepared in the presence of soil.

lipase activity mineral-oil-contamination p-nitrophenyl butyrate soil 

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References

  1. Cooper AB, Morgan HW (1981) Improved fluorimetric method to assay for soil lipase activity. Soil Biol. Biochem. 13: 307-311.Google Scholar
  2. Feller G, Thiry M, Arpigny JL, Gerday C (1991) Cloning and expression in Escherichia coli of three lipase-encoding genes from the psychrotrophic antarctic strain Moraxella TA144. Gene 102: 111-115.Google Scholar
  3. Hankin L, Hill DE, Stephens GR (1982) Effect of mulches on bacterial populations and enzyme activity in soil and vegetable yields. Plant Soil 64: 193-201.Google Scholar
  4. Ishimoto R, Sugimoto M, Kawai F (2001) Screening and characterization of trehalose-oleate hydrolyzing lipase. FEMS Microbiol. Lett. 195: 231-235.Google Scholar
  5. Jaeger KE, Wohlfarth S (1993) Bakterielle Lipasen: Biochemie, Molekulargenetik und biotechnologische Bedeutung. BioEngineering 2: 39-46.Google Scholar
  6. Jaeger KE, Randac S, Dijkstra BW, Colson C, van Heuvel M, Misset O (1994) Bacterial lipases. FEMS Microbiol. Rev. 15: 29-63.Google Scholar
  7. Joga MA, Font X, Gordillo MA, Valero F (2001) Esterase activity by flow injection analysis (FIA). Biotechnol. Lett. 23: 943-948.Google Scholar
  8. Malcolm RE (1983) Assessment of phosphatase activity in soils. Soil Biol. Biochem. 15: 403-408.Google Scholar
  9. Margesin R, Walder G, Schinner F (2000) The impact of hydrocarbon remediation (diesel oil and polycyclic aromatic hydrocarbons) on enzyme activities and microbial properties of soil. Acta Biotechnol. 20: 313-333.Google Scholar
  10. Margesin R, Zimmerbauer A, Schinner F (1999) Soil lipase activity-a useful indicator of oil biodegradation. Biotechnol. Tech. 13: 859-863.Google Scholar
  11. Maurberger A (1987) Methoden zur Bestimmung der Lipaseaktivität von Böden. Bodenlipasehemmung durch Schwermetalle. Master Thesis, University of Innsbruck.Google Scholar
  12. Pancholy SK, Lynd JQ (1972) Quantitative fluorescence analysis of soil lipase activity. Soil Biol. Biochem. 4: 257-259.Google Scholar
  13. Pokorna V (1964) Method of determining the lipolytic activity of upland and lowland peats and muds. Soviet Soil Sci. 1: 85-87.Google Scholar
  14. Quinn DM, Shirai K, Jackson RL, Harmony JAK (1982) Lipoprotein lipase catalyzed hydrolysis of water-soluble p-nitrophenyl esters. Inhibition by apoprotein C-II. Biochemistry 21: 6872-6879.Google Scholar
  15. Schinner F, Öhlinger R, Kandeler E, Margesin R, eds. (1996) Methods in Soil Biology. Berlin: Springer-Verlag.Google Scholar
  16. Shirai K, Jackson RL (1982) Lipoprotein lipase-catalyzed hydrolysis of p-nitrophenyl butyrate. J. Biol. Chem. 257: 1253-1258.Google Scholar
  17. Tabatabai MA (1982) Soil enzymes. In: Page AL, Miller RH, Keeney DR, eds. Methods of Soil Analysis, Part 2. Madison, WI: American Society of Agronomy, pp. 903-947.Google Scholar
  18. Vuorinen AH (1993) Requirement of p-nitrophenol standard for each soil. Soil Biol. Biochem. 25: 295-296.Google Scholar
  19. Zollner H (1989) Handbook of Enzyme Inhibitors. Weinheim: VCH.Google Scholar

Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • R. Margesin
    • 1
  • G. Feller
    • 2
  • M. Hämmerle
    • 1
  • U. Stegner
    • 2
  • F. Schinner
    • 1
  1. 1.Institute of MicrobiologyUniversity of InnsbruckInnsbruckAustria
  2. 2.Laboratory of Biochemistry, Institute of ChemistryUniversity of LiègeLiègeBelgium

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