Plant Growth Regulation

, Volume 34, Issue 2, pp 151–158 | Cite as

The effect of shoot-bending on the amount of diffusible indole-3-acetic acid and its transport in shoots of Japanese pear

  • A. Ito
  • H. Hayama
  • H. Yoshioka


The amount of diffusible indole-3-acetic acid (IAA) in shoots ofJapanese pear (Pyrus pyrifolia) decreased when vertical shootswere bent at an angle of 45°. A significant decrease of diffusibleIAA was observed one day after shoot bending (DAB), and the degree ofthis decrease was larger in the apical region of the shoot than in thebasal region. The decrease caused by the shoot bending increased withthe duration of the treatment. The IAA amounts in the bent shoot in theapical, central, and basal segments on 1 DAB were58.2±6.4%, 92.6±7.6%, and79.1±7.1% of the control, while 43.7±4.1%,30.8±2.9%, and 39.4±2.5% on 14 DAB.Radiolabelled IAA transport velocity was also examined, but it was notinfluenced by the shoot angle in the apical region of the shoot.However, the IAA transport velocity in the basal region decreased. Itdropped first on 1 DAB, but it recovered to the control level 3 DAB,then it decreased again on 14 DAB. A large increase in ethyleneproduction was observed in the bent shoot, but it seemed transient anddid not continue for 14 days. These results suggest that the decrease ofdiffusible IAA amounts may be induced not by the decrease of IAAtransport velocity but by the production/supply of IAA in the apicalregion.

ethylene indole-3-acetic acid Japanese pear Pyrus pyrifolia shoot bending 


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  1. 1.
    Bandurski RS, Cohen JD, Slovin JP and Reinecke DM (1995) Auxin biosynthesis and metabolism. In: Davis PJ (ed) Plant Hormones. Dortrecht: Kluwer Academic Publishers, pp 39–65Google Scholar
  2. 2.
    Bandurski RS, Schulze A and Cohen JD (1977) Photoregulation of the ratio of ester to free indole-3-acetic acid. Biochem Biophys Res Commun 79: 1219–1223Google Scholar
  3. 3.
    Bangerth F (1993) Polar auxin transport as a signal in the regulation of tree and fruit development. Acta Hort 329: 70–76Google Scholar
  4. 4.
    Banno K, Hayashi S and Tanabe K (1985) Effects of SADH and shoot-bending on flower bud formation, nutrient components and endogenous growth regulators in Japanese pear (Pyrus serotina Rehd.). J Japan Soc Hort Sci 53: 365–376 (in Japanese with English abstract and figures)Google Scholar
  5. 5.
    Ben-tal Y and Lavee S (1985) Girdling olive trees, a partial solution to biennial bearing. III Chemical girdling. Rivista Ortoflorofrutt 69: 1–11Google Scholar
  6. 6.
    Cline MG (1994) The role of hormones in apical dominance approaches to an old problem in plant development. Physiol Plant 90: 230–237Google Scholar
  7. 7.
    Cohen JD and Bandurski RS (1982) Chemistry and physiology of the bound auxins. Ann Rev Plant Physiol 33: 403–430Google Scholar
  8. 8.
    Ernest LC and Valdvinos JG (1971) Regulation of auxin levels in Coleus blumei by ethylene. Plant Physiol 48: 402–406Google Scholar
  9. 9.
    Ito A, Hayama H and Yoshioka H (2000) Effect of plant growth regulators on flower bud formation and their fluctuation with application timing in shoot of Japanese pear 'Kosui'. J Japan Soc Hort Sci 69: 536–542Google Scholar
  10. 10.
    Ito A, Yaegaki H, Hayama H, Yamaguchi I, Kusaba S and Yoshioka H (1999) Bending shoots stimulates flowering and influences hormone levels in lateral buds of Japanese pear. HortScience 34: 1224–1228Google Scholar
  11. 11.
    Kaldway H (1984) Transport and other modes of movement of hormones (mainly auxins). In: Scott TK (ed) Hormonal Regulation of Development, II The Function of Hormones from the Level of the Cell to the Whole Plant, Encyclopedia of Plant Physiology (New Series), Vol. 2. Berlin: Springer-Verlag, pp 80–148Google Scholar
  12. 12.
    Kleczkowski K and Schell J (1995) Phytohormone conjugates: nature and function. Critic Rev Plant Sci 14: 283–298Google Scholar
  13. 13.
    Leopold AC, Brown KM and Emerson FH (1972) Ethylene in the wood of stress trees. HortScience 7: 175Google Scholar
  14. 14.
    Li C-J and Bangerth F (1999) Autoinhibition of indoleacetic acid transport in the shoots of two-branched pea (Pisum sativum) plants and its relationship to correlative dominance. Physiol Plant 106: 415–420Google Scholar
  15. 15.
    Lieberman M and Knegt E (1977) Influence of ethylene on indole-3-acetic acid concentration in etiolated pea epicotyl tissues. Plant Physiol 60: 475–477Google Scholar
  16. 16.
    Little CHA and Savidge RA (1987) The role of plant growth regulators in forest tree cambial growth. Plant Growth Regulat 6: 137–169Google Scholar
  17. 17.
    Lomax TL, Muday GK and Rubery PH (1995) Auxin transport. In: Davies PJ (ed) Plant Hormones. Dortrecht: Kluwer Academic Publishers, pp 509–530Google Scholar
  18. 18.
    Luckwill LC (1968) The effect of certain growth regulators on growth and apical dominance of young apple trees. J. Hort Sci 43: 91–101Google Scholar
  19. 19.
    McKoen TA, Fernandez-Maculet JC and Yand S-F (1995) Biosynthesis and metabolism of ethylene. In: Davis PJ (ed) Plant Hormones. Dortrecht: Kluwer Academic Publishers, pp 39–65Google Scholar
  20. 20.
    Momonoki YS (1988) Asymmetric distribution of glucose and indole-3-acetyl-myo-inositol in geostimulated Zea mays seedlings. Plant Physiol 87: 751–756Google Scholar
  21. 21.
    Morgan PW and Drew MC (1997) Ethylene and plant responses to stress. Physiol Plant 100: 620–630Google Scholar
  22. 22.
    Normanly J (1997) Auxin metabolism. Physiol Plant 100: 431–442Google Scholar
  23. 23.
    Parker KE (1991) Auxin metabolism and transport during gravitropism. Physiol Plant 82: 477–482Google Scholar
  24. 24.
    Prasad TK, Hosokawa Z and Cline MG (1989) Shoot inversion-induced ethylene production: A general phenomenon? J Plant Growth Regulat 8: 71–77Google Scholar
  25. 25.
    Sanchez-Bravo J, Ortuno AM, Botia JM, Acosta M and Sabater F (1992) The decrease in auxin polar transport down the lupin hypocotyl could produce the indole-3-acetic acid distribution responsible for the elongation growth pattern. Plant Physiol 99: 108–114Google Scholar
  26. 26.
    Sanyal D and Bangerth F (1998) Stress induced ethylene evolution and its possible relationship to auxin-transport, cytokinin levels, and flower bud induction in shoots of apple seedlings and bearing apple trees. Plant Growth Regulat 24: 127–134Google Scholar
  27. 27.
    Soumelidou K, Morris DA, Battey NH and John P (1994) Auxin transport capacity in relation to the dwarfing effect of apple rootstock. J Hort Sci 69: 719–725Google Scholar
  28. 28.
    Sundberg B and Uggla C (1998) Origin and dynamics of indoleacetic acid under polar transport in Pinus sylvestris. Physiol Plant 104: 22–29Google Scholar
  29. 29.
    Wareing P (1970) Growth and its co-ordination in trees. In: Luckwill LC and Cutting CV (eds) Physiology of Tree Crops. London: Academic Press, pp 1–21Google Scholar
  30. 30.
    Wright M (1982) The polarity of movement of endogenously produced IAA in relation to a gravity perception mechanism. J Exp Bot 33:929–934Google Scholar
  31. 31.
    Yang HM, Ozaki T, Ichii T, Nakanishi T and Kawai Y (1992) Diffusible and extractable auxins in young Japanese pear trees. Scientia Hort 51: 97–106Google Scholar
  32. 32.
    Yang SF and Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Ann Rev Plant Physiol 35: 155–189Google Scholar
  33. 33.
    Yoon IS and Kang BG (1992) Autoregulation of auxin transport in corn coleoptile segments. J Plant Physiol 140: 441–446Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  1. 1.Department of Pomology, National Institute of Fruit Tree ScienceMinistry of Agriculture, Forestry and FisheriesIbarakiJapan
  2. 2.Department of Pomology, National Institute of Fruit Tree ScienceMinistry of Agriculture, Forestry and FisheriesIbarakiJapan
  3. 3.Department of Pomology, National Institute of Fruit Tree ScienceMinistry of Agriculture, Forestry and FisheriesIbarakiJapan

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