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Journal of Radioanalytical and Nuclear Chemistry

, Volume 249, Issue 2, pp 343–348 | Cite as

PIXE application for multi-element analysis of marine micro-algae and measurement of the concentration factor of zinc

  • Y. IwataEmail author
Article

Abstract

Marine micro-algae (Nannochloropsis sp., Phaeodactylum sp., etc.) wereobtained from the Pacific Ocean off Iwate Prefecture, Japan, and culturedpurely in nutritive seawater, PES and artificial seawater, ASP7 as a culturesolution. The algae in 5 ml of the culture solution was collected on a polycarbonatefilm (pore size : 1.0 µm) by suction filtration. The algae on the filmwas subjected to PIXE analysis. A 2.9 MeV proton beam bombarded it at 50 nAfor 15 minutes. Induced X-ray was measured by Si(Li) detector. Concentrationsof Na, Mg, Al, Si, P, S, Cl, K, Ca, Cr, Mn, Fe, Cu, Zn and Sr were determinedsimultaneously. PIXE can used for multi-element analysis of a sample below1 mg, so the culture size for algae was 10–250 ml and every apparatuswas small and of low cost. Nannochloropsis sp. was given a different culturesolution (PES) including Zn2+ from 0.050 to 1.0 mg/l and incubatedat 22 °C, 2000 lux for 10 hours. Zn in Nannochloropsis sp. increased inproportion to the Zn2+ concentration in the culture solution. Theconcentration factor was given as 1600 500 (dry weight base). It was shownthat PIXE was a powerful tool for multi-element analysis of micro organismsand the measurement of bioaccumulation.

Keywords

Pacific Ocean Bioaccumulation Weight Base Proton Beam Concentration Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers/Akadémiai Kiadó 2001

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of Education and Human StudiesAkita UniversityAkitaJapan

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