Advertisement

Journal of Radioanalytical and Nuclear Chemistry

, Volume 249, Issue 2, pp 449–454 | Cite as

Combined biochemical separation and INAA for the determination of iron and other metals in Neuromelanin of human brain Substantia Nigra

  • L. ZeccaEmail author
  • D. Tampellini
  • P. Costi
  • E. Rizzio
  • G. Giaveri
  • M. Gallorini
Article

Abstract

In Parkinson disease Fe and other metals increase in Substantia Nigra(SN) and other basal nuclei. Since Fe can generate citotoxic free radicals,Neuromelanin (NM) could play an important protective role in neurons. In thiswork an original procedure for separation of NM, preparation of samples andanalysis is presented. The determination of SN and its NM elemental contentwas carried out by instrumental neutron activation analysis. Several actionswere taken to reduce the metal contaminations: use of high purity reagents,dissection of tissues with titanium coated tools and adequate processing ofsamples.

Keywords

Titanium Free Radical Human Brain Activation Analysis Protective Role 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    R. Carstam,C. Brink,A. Hindemint-Augustsson,H. Rorsman,E. Rosengen, Biochem. Biophys. Acta, 1097 (1991) 157.Google Scholar
  2. 2.
    B. Bogert, J. Comp. Neurol., 197 (1981) 63.CrossRefGoogle Scholar
  3. 3.
    D. M. A. Mann,P. O. Yates, Brain, 97 (1974) 489.CrossRefGoogle Scholar
  4. 4.
    S. Aime,B. Fasano,B. Bergamasco,L. Lopiano,G. Valente, J. Neurochem., 62 (1994) 369.CrossRefGoogle Scholar
  5. 5.
    M. H. Van Woert,K. N. Prasad,D. C. Borg, J. Neurochem., 14 (1967) 707.CrossRefGoogle Scholar
  6. 6.
    L. Zecca,E. Parati,C. Mecacci,R. Seraglia, Biochim. Biophys. Acta, 1138 (1992) 6.CrossRefGoogle Scholar
  7. 7.
    W. S. Enochs,T. Sarna,L. Zecca,P. A. Riley,H. M. Swartz, J. Neural Transm. Park. Dis. Dement., Sect. 7 (1994) 83.Google Scholar
  8. 8.
    M. Gerlach,A. X. Trautwein,L. Zecca,M. B. H. Youdim,P. Riederer, J. Neurochem., 65 (1995) 923.CrossRefGoogle Scholar
  9. 9.
    H. M. Swartz,T. Sarna,L. Zecca, Ann. Neurol., 32 (1992) S69.CrossRefGoogle Scholar
  10. 10.
    L. Zecca,R. Pietra,C. Goj,C. Mecacci,D. Radice,E. Sabbioni, J. Neurochem., 62 (1994) 1097.CrossRefGoogle Scholar
  11. 11.
    D. Ben-Shachar,P. Riederer,M. B. H. Youdim, J. Neurochem., 57 (1991) 1609.CrossRefGoogle Scholar
  12. 12.
    K. Jellinger,E. Kienzl,G. Rumpelmaier,P. Riederer,H. Stackelberger,D. Ben-Shachar,M. B. H. Youdim, J. Neurochem., 59 (1992) 1168.CrossRefGoogle Scholar
  13. 13.
    T. Sarna,W. Korytowsky,M. Zareba,L. Zecca, Pigment Cell Res., 6 (1993) 287.Google Scholar
  14. 14.
    P. F. Good,C. W. Olanow,D. P. Perl, Brain Res., 593 (1992) 343.CrossRefGoogle Scholar
  15. 15.
    A. J. Kropf,B. A. Bunker,M. Elsner,S. C. Moss,L. Zecca,A. Stroppolo,P. R. Crippa, Biophys. J., 75 (1998) 3135.CrossRefGoogle Scholar
  16. 16.
    M. Zareba,A. Bober,W. Korytowski,L. Zecca,T. Sarna, Biochim. Biophys. Acta, 1271 (1995) 343.CrossRefGoogle Scholar
  17. 17.
    R. Pietra,E. Sabbioni,F. Brossa,M. Gallorini,G. Faruffini,E. M. Fumagalli, Analyst, 115 (1990) 1025.CrossRefGoogle Scholar
  18. 18.
    L. Zecca,T. Shima,A. Stroppolo,C. Goj,G. A. Battiston,R. Gerbasi,T. Sarna,H. M. Swartz, Neurosci., 73 (1996) 407.CrossRefGoogle Scholar
  19. 19.
    M. G. Bridelli,D. Tampellini,L. zecca, Febs Letters, 457 (1999) 18.CrossRefGoogle Scholar
  20. 20.
    W. R. Markesbery,W. D. Ehmann,M. Alauddin,T. I. M. Hossain, Neurobiol. Aging, 5 (1984) 19.CrossRefGoogle Scholar
  21. 21.
    P. Riederer,E. Sofic,W. Rausch,B. Schmidt,G. P. Reynolds,K. Jellinger,M. B. H. Youdim, J. Neurochem., 52 (1989) 515.CrossRefGoogle Scholar
  22. 22.
    E. Sofic,P. Riederer,H. Heinsen,H. Beckmann,C. P. Reynolds,G. Hebenstreit,M. B. H. Youdim, J. Neural Transm., 74 (1988) 199.CrossRefGoogle Scholar
  23. 23.
    D. T. Dexter,F. R. Wells,A. J. Lees,Y. Agid,P. Jenner,C. D. Marsden, J. Neurochem., 52 (1989) 1830.CrossRefGoogle Scholar
  24. 24.
    J. Galazka-Friedman,E. R. Bauminger,A. Friedman,M. Barcikowska,D. Hechel,I. Nowik, Mov. Disord., 11 (1996) 8.CrossRefGoogle Scholar
  25. 25.
    T. Sarna,J. S. Hyde,H. M. Swartz, Science, 192 (1976) 1132.CrossRefGoogle Scholar
  26. 26.
    J. M. Gorell,C. C. Johnson,B. A. Rybicki,E. L. Peterson,G. X. Kortsha,G. G. Brown,R. J. Richardson, Neurotoxicology, 20 (1999) 239.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers/Akadémiai Kiadó 2001

Authors and Affiliations

  • L. Zecca
    • 1
    Email author
  • D. Tampellini
    • 1
  • P. Costi
    • 1
  • E. Rizzio
    • 2
  • G. Giaveri
    • 2
  • M. Gallorini
    • 2
  1. 1.Institute of Advanced Biomedical Technologies-CNRSegrate- MilanoItaly
  2. 2.Center of Radiochemistry and Activation Analysis-CNRPaviaItaly

Personalised recommendations