Methods in Cell Science

, Volume 23, Issue 1–3, pp 141–150 | Cite as

Molecular cytogenetics of introgressive hybridization in plants

  • Kesara Anamthawat-Jónsson
Article

Abstract

Introgressive hybridization (introgression) is genetic modification of one species by another through hybridization and repeated backcrossing. Introgression is important in the evolution of flowering plants. It is also important in plant breeding where a desirable trait can be transferred from wild to crop species. One of the most recent advances in molecular techniques for studying hybridization and introgression is in situ hybridization of genomic probes to cytological preparations (GISH, genomic in situ hybridization). The present paper describes a successful GISH protocol for detection of intergenomic introgression in breeding materials and in allopolyploid species. In addition, the paper introduces a new possibility of using dispersed repeats to detect introgression and to gain insights into its molecular basis. The approach is referred to as dFISH for dispersed fluorescence in situ hybridization, and the best candidate for this type of probes is probably a retroelement. Southern hybridization data are also presented to support the effectiveness of GISH and dFISH for introgression mapping.

Dispersed fluorescence in situ hybridization (dFISH) Genomic in situ hybridization (GISH) Introgressive hybridization (introgression) Plant breeding Plant evolution 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anamthawat-Jónsson K, Bödvarsdóttir SK (1998). Meiosis of wheat × lymegrass hybrids. Chromosome Res 6: 339–343.Google Scholar
  2. 2.
    Anamthawat-Jónsson K, Heslop-Harrison JS (1993). Isolation and characterization of genome-specific DNA sequences in Triticeae species. Mol Gen Genet 240: 151–158.Google Scholar
  3. 3.
    Anamthawat-Jónsson K, Heslop-Harrison JS (1996). Establishing relationships between closely related species using total genomic DNA as a probe. In: Clapp JP (ed), Methods in molecular biology, vol. 50. New Jersey: Humana Press, pp 209–225.Google Scholar
  4. 4.
    Anamthawat-Jónsson K, Reader SM (1995). Pre-annealing of total genomic DNA probes for simultaneous in situ hybridization in cereal species. Genome 38: 814–816.Google Scholar
  5. 5.
    Anamthawat-Jónsson K, Schwarzacher T, Leitch AR et al. (1990). Discrimination between closely related Triticeae species using genomic DNA as a probe. Theor Appl Genet 79: 721–728.Google Scholar
  6. 6.
    Anamthawat-Jónsson K, Thórsson ÆTh, Salmela E (2001). Molecular and genomic evidence for introgressive hybridization in birch. J Hered.Google Scholar
  7. 7.
    Anderson E (1949). Introgressive hybridization. London: Chapman and Hall.Google Scholar
  8. 8.
    Bennetzen JL (1998). The structure and evolution of angiosperm nuclear genomes. Curr Opin Plant Biol 1: 103–108.Google Scholar
  9. 9.
    Briggs D, Walters SM (1997). Plant variation and evolution. Cambridge: Cambridge University Press.Google Scholar
  10. 10.
    Brar DS, Khush GS (1997). Alien introgression in rice. Plant Mol Biol 35: 35–47.Google Scholar
  11. 11.
    Busch W, Hermann RG, Houben A et al. (1996). Efficient preparation of plant metaphase spreads. Plant Mol Biol Rep 14: 149–155.Google Scholar
  12. 12.
    Chen Q, Armstrong K (1994). Genomic in situ hybridization in Avena sativa. Genome 37: 607–612.Google Scholar
  13. 13.
    Chevre AM, Eber F, Baranger A et al. (1997). Gene flow from transgenic crops. Nature 389: 924.Google Scholar
  14. 14.
    Chevre AM, Eber F, Baranger A et al. (1998). Characterization of backcross generation obtained under field conditions from oilseed rape-wild radish F1 interspecific hybrids: an assessment of transgene dispersal. Theor Appl Genet 97: 90–98.Google Scholar
  15. 15.
    Ellneskog-Staam P, Merker A. Genome composition, stability and fertility of amphidiploids between Triticum turgidum var. carthlicum and Leymus racemosus. Genome.Google Scholar
  16. 16.
    Ellneskog-Staam P, von Bothmer R, Anamthawat-Jónsson K (2001). Trigenomic origin of the hexaploid Psammopyrum athericum (Triticeae: Poaceae) revealed by in situ hybridization. Chromosome Res 9: 243–249.Google Scholar
  17. 17.
    Ellstrand NC, Whitkus RW, Rieseberg LH (1996). Distribution of spontaneous plant hybrids. Proc Natl Acad Sci USA 93: 5090–5093.Google Scholar
  18. 18.
    Ellstrand NC, Prentice HC, Hancock JF (1999). Gene flow and intyrogression from domesticated plants into their wild species. Annu Rev Ecol Syst 30: 539–563.Google Scholar
  19. 19.
    Frello S, Hansen KR, Jensen J et al. (1995). Inheritance of rapeseed (Brassica napus) specific RAPD markers and a transgene in the cross B. juncea × (B. juncea. × B. napus). Theor Appl Genet 91: 236–241.Google Scholar
  20. 20.
    Friebe B, Jiang J, Gill BS et al. (1993). Radiation-induced nonhomoeologous wheat-Agropyron intermedium chromosomal translocations conferring resistance to leaf rust. Theor Appl Genet 86: 141–149.Google Scholar
  21. 21.
    Garriga-Caldere F, Huigen DJ, Jacobsen E et al. (1999). Prospects for introgressing tomato chromosomes into the potato genome: an assessment through GISH analysis. Genome 42: 282–288.Google Scholar
  22. 22.
    Gill BS, Friebe B (1998). Plant cytogenetics at the dawn of the 21st century. Curr Opin Plant Biol 1: 109–115.Google Scholar
  23. 23.
    Hauser TP, Jorgensen RB, Ostergard H (1998). Fitness of backcross and F2 hybrids between weedy Brassica rapa and oilseed rape (B. napus). Heredity 81: 436–443.Google Scholar
  24. 24.
    Heiser CB (1973). Introgression re-examined. Bot Rev 39: 347–366.Google Scholar
  25. 25.
    Heslop-Harrison JS, Leitch AR, Schwarzacher T et al. (1990). Detection and characterization of 1B/1R translocations in hexaploid wheat. Heredity 65: 385–392.Google Scholar
  26. 26.
    Humphreys MW, Pasakinskiene I (1996). Chromosome painting to locate genes for drought resistance transferred from Festuca arundinacea into Lolium multiflorum. Heredity 77: 530–534.Google Scholar
  27. 27.
    Humphreys M, Thomas H-M, Harper J et al. (1997). Dissecting drought-and cold-tolerance traits in the Lolium-Festuca complex by introgression mapping. New Phytol 137: 55–60.Google Scholar
  28. 28.
    Jarvis DI, Hodgkin T (1999). Wild relatives and crop cultivars: detecting natural introgression and farmer selection of new genetic combinations in agroecosystem. Mol Ecol 8: S159–S173.Google Scholar
  29. 29.
    Jauhar PP, Chibbar RN (1999). Chromosome-mediated and direct gene transfers in wheat. Genome 42: 570–583.Google Scholar
  30. 30.
    Jiang J, Gill BS (1994). Nonisotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37: 717–725.Google Scholar
  31. 31.
    Jiang J, Friebe B, Gill BS (1994). Recent advances in alien gene transfer in wheat. Euphytica 73: 199–212.Google Scholar
  32. 32.
    Kenton A, Parokonny AS, Gleba YY et al. (1993). Characterization of the Nicotiana tabacum L. genome by molecular cytogenetics. Mol Gen Genet 240: 159–169.Google Scholar
  33. 33.
    Lashermes P, Andrzejewski S, Bertrand B et al. (2000). Molecular analysis of introgressive breeding in coffee. Theor Appl Genet 100: 139–146.Google Scholar
  34. 34.
    Le HT, Armstrong KC, Miki B (1989). Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe. Plant Mol Biol Rep 7: 150–158.Google Scholar
  35. 35.
    Leitch IJ, Bennett MD (1997). Polyploidy in angiosperms. Trneds Plant Sci 2: 470–476.Google Scholar
  36. 36.
    Miller TE, Reader SM, Purdie KA et al. (1995). Fluorescent in situ hybridization as an aid to introducing alien genetic variation into wheat. Euphytica 85: 275–279.Google Scholar
  37. 37.
    Morrison LA, Cremieux L, Zemetra RS et al. (2000). Gene flow in the crop-weed complex of wheat (Triticum aestivum L.) and jointed goatgrass (Aegilops cylindrica Host). Amer J Bot 87: 59.Google Scholar
  38. 38.
    Ørgaard M, Anamthawat-Jónsson K (2001). Genome discrimination by in situ hybridization in Icelandic species of Elymus and Elytrigia (Poaceae: Triticeae). Genome 44: 275–283.Google Scholar
  39. 39.
    Pasakinskiene I, Anamthawat-Jónsson K, Humphreys MW et al. (1998). New molecular evidence on genome relationships and chromosome identification in Festuca and Lolium. Heredity 81: 659–665.Google Scholar
  40. 40.
    Pickering RA, Malyshev S, Kunzel G et al. (2000). Locating introgressions of Hordeum bulbosum chromatin within the H. vulgare genome. Theor Appl Genet 100: 27–31.Google Scholar
  41. 41.
    Rhymer JM, Simberloff D (1996). Extinction by hybridization and introgression. Annu Rev Ecol Syst 27: 83–109.Google Scholar
  42. 42.
    Rieseberg LH (1997). Hybrid origins of plant species. Annu Rev Ecol Syst 28: 359–389.Google Scholar
  43. 43.
    Rieseberg LH, Wendel J (1993). Introgression and its consequences in plants. In: Harrison R (ed), Hybrid zones and the evolutionary process. New York: Oxford University Press, pp 70–109.Google Scholar
  44. 44.
    Rieseberg LH, Baird SJE, Gardner KA (2000). Hybridization, introgression, and linkage evolution. Plant Mol Biol 42: 205–224.Google Scholar
  45. 45.
    Schwarzacher T, Leitch AR (1994). Enzymatic treatment of plant material to spread chromosomes for in situ hybridization. In: Isaac PJ (ed), Methods in molecular biology 28: Protocols for nucleic acid analysis by nonradioactive probes. New Jersey: Humana Press, pp 153–160.Google Scholar
  46. 46.
    Schwarzacher T, Leitch AR, Bennett MD et al. (1989). In situ localization of parental genomes in a wide hybrid. Ann Bot 64: 315–324.Google Scholar
  47. 47.
    Schwarzacher T, Anamthawat-Jónsson K, Harrison GE et al. (1992). Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat. Theor Appl Genet 84: 778–786.Google Scholar
  48. 48.
    Skarzhinskaya M, Fahleson J, Glimelius K et al. (1998). Genome organization of Brassica napus and Lesquerella fendleri and analysis of their somatic hybrids using genomic in situ hybridization. Genome 41: 691–701.Google Scholar
  49. 49.
    Snow AA, Andersen B, Jorgensen RB (1999). Costs of transgenic herbicide resistance introgressed from Brassica napus into weedy B. rapa. Mol Ecol 8: 605–615.Google Scholar
  50. 50.
    Snowdon RJ, Köhler W, Friedt W et al. (1997). Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids. Theor Appl Genet 95: 1320–1324.Google Scholar
  51. 51.
    Suoneimi A, Anamthawat-Jónsson K, Arna T et al. (1996). Retrotransposon BARE-1 is a major component of the barley (Hordeum vulgare L.) genome. Plant Mol Biol 30: 1321–1329.Google Scholar
  52. 52.
    Takeda S, Ando H, Takeda K et al. (1999). Detection of Hordeum marinum genome in three polyploid Hordeum species and cytotypes by genomic in situ hybridizaiton. Hereditas 130: 185–188.Google Scholar
  53. 53.
    Vicient CM, Suoniemi A, Anamthawat-Jónsson K et al. (1999). Retrotransposon BARE-1 and its role in genome evolution in Hordeum. Plant Cell 11: 1769–1784.Google Scholar
  54. 54.
    Wendel JF, Wessler SR (2000). Retrotransposon-mediated genome evolution on a local ecological scale. Proc Natl Acad Sci USA 97: 6250–6252.Google Scholar
  55. 55.
    Young ND, Tanksley SD (1989). Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet 77: 95–101.Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Kesara Anamthawat-Jónsson
    • 1
  1. 1.Department of BiologyUniversity of IcelandReykjavíkIceland

Personalised recommendations