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Aerobiologia

, Volume 17, Issue 4, pp 313–318 | Cite as

Possibility of real-time measurement of an airborne Cryptomeria japonica pollen allergen based on the principle of surface plasmon resonance

  • Yuichi Takahashi
  • Takeshi Ohashi
  • Takao Nagoya
  • Masahiro Sakaguchi
  • Hiroshi Yasueda
  • Hiroshi Nitta
Article

Abstract

In order to establish a real-timemeasurement system for the concentration ofairborne pollen allergens, we examined Cry j 1,one of the major allergens of Cryptomeriajaponica pollen, as an example to establishthe system. The feasible system consisted of:collection of airborne pollen allergens usingthe Virtual Impactor or Cyclone sampler,extraction of Cry j 1 using 10mM HEPES buffercontaining 0.125M NH4HCO3 andfinally, real-time measurement of Cry j 1 usingthe BIACORE 3000 system. The sensitivity of thesystem was 5 ng/ml, and 0.1 ml sample volumeand at least 500 pg of Cry j 1 were requiredfor each measurement. Quantification of Cry j 1in the air can be determined 30 min aftercollection, i.e. 15 min for extraction, 10 minfor separation from particulate matters and 5min for the measurement.

airborne pollen Cryptomeria pollen pollen allergen real-time measurement SPR 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Yuichi Takahashi
    • 1
  • Takeshi Ohashi
    • 2
  • Takao Nagoya
    • 3
  • Masahiro Sakaguchi
    • 4
  • Hiroshi Yasueda
    • 5
  • Hiroshi Nitta
    • 6
  1. 1.Yamagata Pref. Inst. Pub. HealthYamagataJapan
  2. 2.Biacore K.K.TokyoJapan
  3. 3.Kowa Research Inst. Kowa, Co. Ltd.IbarakiJapan
  4. 4.Natl. Inst. Infectious DiseasesTokyoJapan
  5. 5.Sagamihara Natl. HospitalKanagawaJapan
  6. 6.Natl. Inst. Environmental StudiesIbarakiJapan

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