Biotechnology Letters

, Volume 23, Issue 22, pp 1889–1892 | Cite as

Analysis of the methane production in thermophilic anaerobic reactors: use of autofluorescence microscopy

  • R. Solera
  • L.I. Romero
  • D. Sales


Methanogenic activity in thermophilic, anaerobic reactors was determined by comparing the amount of methane generated in single- and two-stage systems with the size of the methanogenic population, as determined by microscopy. The methanogenic activities were 2.71 × 10−9 ml methane cell−1 d−1 and 1.10 × 10−9 ml methane cell−1 d−1 for 10 and 4 days of the hydraulic retention time (HRT), in the single-stage system. In the two-stage system, 7.49 × 10−9 ml methane cell−1 d−1 in the acidogenic reactor and 1.56 × 10−9 ml methane cell−1 d−1 in the methanogenic reactor for 4 days of the HRT. A high correlation was evident between the methane production and methanogenic population [0.1354 ln(x) − 2.1375](R2 0.8619).

activity anaerobic process biogas methanogens waste water treatment 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • R. Solera
    • 1
  • L.I. Romero
    • 1
  • D. Sales
    • 1
  1. 1.Department of Chemical Engineering, Food and Environmental Technologies, Faculty of Marine and Environmental SciencesUniversity of Cadiz, Campus Río San Pedro s/nCadizSpain

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