Euphytica

, Volume 122, Issue 1, pp 191–201 | Cite as

AFLP analysis of genetic diversity within and between populations of perennial ryegrass (Lolium perenne L.)

  • K.M. Guthridge
  • M.P. Dupal
  • R. Kölliker
  • E.S. Jones
  • K.F. Smith
  • J.W. Forster
Article

Abstract

Amplified fragment length polymorphism (AFLP) analysis has been used to measure genetic diversity in perennial ryegrass (Lolium perenne L.) and to relate intra- and interpopulation variation to breeding history. Cluster analysis of AFLP data from contrasting populations showed features consistent with the origins of these varieties. Significant differences in intrapopulation diversity were detected and partial separation of different cultivars was observed. Restricted base cultivars, derived from small numbers of foundation clones, were suitable for this type of study, allowing near complete discrimination of closely related cultivars. Analysis of bulked samples was based on the pooling of genomic DNA from 20 individuals from 6 selected populations. Cluster analysis of AFLP data from bulked samples produced a phenogram showing relationships consistent with the results of individual analysis. AFLP profiling provides an important tool for the detection and quantification of genetic variation in perennial ryegrass.

AFLP phenogram genetic diversity molecular marker Lolium perenne L. 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • K.M. Guthridge
    • 1
    • 2
  • M.P. Dupal
    • 1
    • 2
  • R. Kölliker
    • 1
  • E.S. Jones
    • 1
    • 2
  • K.F. Smith
    • 3
    • 2
  • J.W. Forster
    • 1
    • 2
  1. 1.Plant Biotechnology Centre, Agriculture VictoriaLa Trobe UniversityBundooraAustralia
  2. 2.CRC for Molecular Plant BreedingAustralia
  3. 3.Pastoral and Veterinary InstituteAgriculture Victoria HamiltonHamiltonAustralia

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