Plant Molecular Biology

, Volume 47, Issue 5, pp 677–692

Expression and evolution of functionally distinct haemoglobin genes in plants

  • P.W. Hunt
  • R.A. Watts
  • B. Trevaskis
  • D.J. Llewelyn
  • J. Burnell
  • E.S. Dennis
  • W.J. Peacock
Article

Abstract

Haemoglobin genes have been found in a number of plant species, but the number of genes known has been too small to allow effective evolutionary inferences. We present nine new non-symbiotic haemoglobin sequences from a range of plants, including class 1 haemoglobins from cotton, Citrus and tomato, class 2 haemoglobins from cotton, tomato, sugar beet and canola and two haemoglobins from the non-vascular plants, Marchantia polymorpha (a liverwort) and Physcomitrella patens (a moss). Our molecular phylogenetic analysis of all currently known non-symbiotic haemoglobin genes and a selection of symbiotic haemoglobins have confirmed the existence of two distinct classes of haemoglobin genes in the dicots. It is likely that all dicots have both class 1 and class 2 non-symbiotic haemoglobin genes whereas in monocots we have detected only class 1 genes. The symbiotic haemoglobins from legumes and Casuarina are related to the class 2 non-symbiotic haemoglobins, whilst the symbiotic haemoglobin from Parasponia groups with the class 1 non-symbiotic genes. Probably, there have been two independent recruitments of symbiotic haemoglobins. Although the functions of the two non-symbiotic haemoglobins remain unknown, their patterns of expression within plants suggest different functions. We examined the expression in transgenic plants of the two non-symbiotic haemoglobins from Arabidopsis using promoter fusions to a GUS reporter gene. The Arabidopsis GLB1 and GLB2 genes are likely to be functionally distinct. The class 2 haemoglobin gene (GLB2) is expressed in the roots, leaves and inflorescence and can be induced in young plants by cytokinin treatment in contrast to the class 1 gene (GLB1) which is active in germinating seedlings and can be induced by hypoxia and increased sucrose supply, but not by cytokinin treatment.

Arabidopsis cytokinin evolution haemoglobin nitrogen-fixing symbiosis reporter-gene expression 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • P.W. Hunt
    • 1
  • R.A. Watts
    • 2
  • B. Trevaskis
    • 1
  • D.J. Llewelyn
    • 1
  • J. Burnell
    • 3
  • E.S. Dennis
    • 1
  • W.J. Peacock
    • 1
  1. 1.CSIRO Division of Plant IndustryCanberraAustralia
  2. 2.School of Biochemistry and Molecular BiologyAustralian National UniversityCanberraAustralia
  3. 3.School of Molecular SciencesJames Cook UniversityTownsvilleAustralia

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