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Biotechnology Letters

, Volume 23, Issue 20, pp 1697–1702 | Cite as

Synchronous luminescence: a simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein

  • Minoo Askari
  • Gordon Miller
  • Tuan Vo-Dinh
Article

Abstract

Human fragile histidine triad (FHIT) protein has dinucleoside 5′,5′′′-P1,Pn-polyphosphates hydrolysis activity, with AMP being one of the reaction products. Application of synchronous luminescence (SL) spectroscopy, in which both excitation and emission wavelengths are scanned simultaneously while a constant wavelength interval is maintained between them, was investigated for detection of the enzymatic activity of the FHIT protein. Ability of SL to identify reaction components, AMP production and its increase as a result of increase in substrate concentration and inhibition of the hydrolysis activity by ZnCl2 are demonstrated.

fragile histidine triad gene fluorescence spectroscopy hydrolysis synchronous luminescence spectroscopy 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Minoo Askari
    • 1
  • Gordon Miller
    • 1
  • Tuan Vo-Dinh
    • 1
  1. 1.Advanced Monitoring Development Group, Life Sciences DivisionOak Ridge National LaboratoryOak RidgeUSA

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