Biochemistry (Moscow)

, Volume 66, Issue 9, pp 984–988 | Cite as

Activation of the Escherichia coli SoxRS-Regulon by Nitric Oxide and Its Physiological Donors

  • S. V. Vasil'eva
  • M. V. Stupakova
  • I. I. Lobysheva
  • V. D. Mikoyan
  • A. F. Vanin


Activation of the Escherichia coli SoxRS-regulon by nitric oxide (NO) and its physiological donors (S-nitrosothiol (GS-NO) and dinitrosyl iron complexes with glutathione (DNICglu) and cysteine (DNICcys) ligands) has been studied. To elucidate the molecular mechanisms of signal transduction via nitrosylation of Fe-S-centers in SoxR, the ability of pure NO and NO-producing agents to activate the SoxRS-regulon in E. coli cells bearing a soxS::lacZ operon (promoter) fusion has been compared. EPR spectroscopy of whole cells has been used to monitor the formation of inducible protein–DNIC complexes. DNICcys, GS-NO, and pure NO appeared to be potent inducers of soxS expression, whereas DNICglu was considerably less efficient. Thus, lower in vitro stability of DNICcys was in contrast with its higher biological activity. Pretreatment of the cells with o-phenanthroline, a chelating agent for iron, prevented soxS expression by GS-NO. Treatment of intact E. coli cells with DNIC, GS-NO, and NO at equimolar concentration 150 μM resulted in formation of a single EPR-detectable DNIC-type signal with g = 2.03. The initial stage in the SoxR transcription activity is supposed to include two steps: first, DNIC primers are formed from exogenous NO and free iron, and then these DNIC disintegrate SoxR [2Fe-2S] clusters and thus activate SoxRS-regulon transcription.

nitric oxide SoxRS-regulon S-nitrosothiols dinitrosyl iron complexes Escherichia coli 


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  1. 1.
    Hidalgo, E., Bollinger, Jr., Bradley, T. M., Walsh, C. T., and Demple, B. (1995) J.Biol.Chem., 270, 20908–20915.PubMedGoogle Scholar
  2. 2.
    Demple, B. (1999) Clinic.Exp.Pharmacol.Physiol., 26, 64–73.Google Scholar
  3. 3.
    Greenberg, J. T., and Demple, B. (1995) J.Bacteriol., 178, 1154–1163.Google Scholar
  4. 4.
    Chou, J. H., Greenberg, J. T., and Demple, B. (1993) J.Bacteriol., 175, 1026–1034.PubMedGoogle Scholar
  5. 5.
    Demple, B. (1996) Gene, 179, 53–64.PubMedGoogle Scholar
  6. 6.
    Farr, S. B., and Kogoma, T. (1991) Microbiol.Rev., 55, 561–572.PubMedGoogle Scholar
  7. 7.
    Tsaneva, I. R., and Weiss, B. (1990) J.Bacteriol., 172, 4197–4205.PubMedGoogle Scholar
  8. 8.
    Hidalgo, E., and Demple, B. (1996) in Regulation of Gene Expression in Escherichia coli(Lin, E., and Lynch, A., eds.) R. G. Landers, Austin, TX, pp. 435–452.Google Scholar
  9. 9.
    Demple, B. (1999) Brazil.J.Med.Biol.Res., 32, 1417–1427.Google Scholar
  10. 10.
    Kopenol, W. H., Kissner, R., and Beckman, J. S. (1996) Meth.Enzymol., 269, 296–302.PubMedGoogle Scholar
  11. 11.
    Nunoshiba, T., de-Rojas-Walker, T., Wishnok, S., Tannenbaum, S., and Demple, B. (1993) Proc.Natl.Acad.Sci.USA, 90, 9993–9997.PubMedGoogle Scholar
  12. 12.
    Miller, J. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.Google Scholar
  13. 13.
    Ding, H., and Demple, B. (1998) Biochemistry, 37, 17280–17286.PubMedGoogle Scholar
  14. 14.
    Ding, H., and Demple, B. (2000) Proc.Natl.Acad.Sci.USA, 97, 5146–5150.CrossRefPubMedGoogle Scholar
  15. 15.
    Stupakova, M. V., Lobysheva, I. I., Mikoyan, V. D., Vanin, A. F., and Vasil.eva, S. V. (2000) Biochemistry (Moscow), 65, 690–695.Google Scholar
  16. 16.
    Vanin, A. F., Stukan, R. A., and Manukhina, E. B. (1996) Biochim.Biophys.Acta, 1295, 5–12.PubMedGoogle Scholar
  17. 17.
    Vanin, A. F. (1998) Biochemistry (Moscow), 63, 782–793.Google Scholar
  18. 18.
    Voevodskaya, N. V., Kubrina, L. N., Serezhenkov, V. A., Mikoyan, V. D., and Vanin, A. F. (1999) Curr.Top.Biophys., 23, 31–38.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2001

Authors and Affiliations

  • S. V. Vasil'eva
    • 1
  • M. V. Stupakova
    • 1
  • I. I. Lobysheva
    • 1
  • V. D. Mikoyan
    • 1
  • A. F. Vanin
    • 1
  1. 1.Russian Academy of SciencesInstitute of Biochemical PhysicsRussia

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