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Journal of Protein Chemistry

, Volume 20, Issue 5, pp 367–375 | Cite as

Isolation of a Homodimeric Lectin with Antifungal and Antiviral Activities from Red Kidney Bean (Phaseolus vulgaris) Seeds

  • X. Y. Ye
  • T. B. Ng
  • Paul W. K. Tsang
  • J. Wang
Article

Abstract

A homodimeric lectin adsorbed on Affi-gel blue gel and CM-Sepharose and possessing a molecular weight of 67 kDa was isolated from red kidney beans. The hemagglutinating activity of this lectin was inhibited by glycoproteins but not by simple sugars. The lectin manifested inhibitory activity on human immunodeficiency virus-1 reverse transcriptase and α-glucosidase. The N-terminal sequence of the lectin exhibited some differences from previously reported lectins from Phaseolus vulgaris but showed some similarity to chitinases. It exerted a suppressive effect on growth of the fungal species Fusarium oxysporum, Coprinus comatus, and Rhizoctonia solani. The lectin had low ribonuclease and negligible translation-inhibitory activities.

Lectin red kidney beans antifungal antiviral 

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Copyright information

© Plenum Publishing Corporation 2001

Authors and Affiliations

  • X. Y. Ye
    • 1
  • T. B. Ng
    • 1
  • Paul W. K. Tsang
    • 1
  • J. Wang
    • 1
  1. 1.Department of BiochemistryThe Chinese University of Hong KongShatin, N.T.Hong Kong, China

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