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Antonie van Leeuwenhoek

, Volume 80, Issue 1, pp 57–63 | Cite as

Rapid flow cytometry – Nile red assessment of PHA cellular content and heterogeneity in cultures of Pseudomonas aeruginosa 47T2 (NCIB 40044) grown in waste frying oil

  • J. Vidal-Mas
  • O. Resina-Pelfort
  • E. Haba
  • J. Comas
  • A. Manresa
  • J. Vives-RegoEmail author
Article

Abstract

The accumulation of cytoplasmic polyhydroxyalkanoates (PHAs) and the heterogeneity of bacterial populations were analysed by flow cytometry and SYTO-13 and Nile red staining in rhamnolipid-producing Pseudomonas aeruginosa cultures grown in waste frying oil as carbon source. A combination of SYTO-13 and Nile red fluorescence with cytometric forward and side scatter values may allow increases in the final production of polyhydroxyalkanoates (PHA) by two basic mechanisms: (i) rapid assessment of polyhydroxyalkanoate content and (ii) definition of flow cytometric cell sorting protocols to select high polyhydroxyalkanoate (PHA)-producing strains. We report a rapid (less than 30 min) flow cytometric assessment of PHAs in Pseudomonas aeruginosa 47T2 following Nile red staining: (i) to estimate cellular PHAs content; (ii) to study heterogeneity of the batch cultures producing PHAs and (iii) to establish the basis for sorting sub-populations with a high capacity to accumulate PHAs.

flow cytometry Nile red PHA Pseudomonas aeruginosa rhamnolipids SYTO-13 waste frying oils 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • J. Vidal-Mas
    • 1
  • O. Resina-Pelfort
    • 2
  • E. Haba
    • 1
  • J. Comas
    • 3
  • A. Manresa
    • 1
  • J. Vives-Rego
    • 2
    Email author
  1. 1.Departament de Microbiologia i Parasitologia Sanitàries, Facultat de FarmaciaUniversitat de BarcelonaBarcelonaSpain
  2. 2.Departament de MicrobiologiaUniversitat de BarcelonaBarcelona
  3. 3.Servei Científico TècnicUniversitat de BarcelonaBarcelonaSpain

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