Euphytica

, Volume 121, Issue 3, pp 349–356 | Cite as

Barley somaclones associated with high yield or resistance to powdery mildew

  • J.C. Li
  • T.M. Choo
  • K.M. Ho
  • D.E. Falk
  • R. Blatt

Abstract

A study was conducted to evaluate the usefulness of somaclonal variation as a means to obtain powdery mildew resistance in the background of an agronomically elite, high-yielding barley (Hordeum vulgare L.) cultivar. A total of 170 Ro-derived lines were regenerated from embryo-induced callus of the barley cultivar Léger. Forty-five lines were selected and evaluated in replicated field plots at two locations in Eastern Canada. In comparison with Léger, one of the 45 lines was higher yielding, one produced a greater test weight, two had a greater seed weight, and one was shorter in plant height. Three lines were found to segregate for resistance to powdery mildew (Erysiphe graminis DC ex Merat f. sp. hordei EM). Many of the single-plant selections from the three resistant lines showed resistance to powdery mildew under field conditions for two years. Three lines were eventually promoted to the official registration tests in Ontario. One of the three lines was subsequently registered as a new cultivar (AC Malone) in Canada. To our knowledge, AC Malone is the world's first barley cultivar selected from somaclonal variation. The results of this study demonstrate that it is possible to obtain high- yielding or disease-resistant lines from callus culture in barley.

barley high yield Hordeum vulgare powdery mildew somaclonal variation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ahloowalia, B.S., 1987. Plant regeneration from embryo-callus culture in barley. Euphytica36: 659–665.CrossRefGoogle Scholar
  2. Baillie, A.M.R, B.G. Rossnagel & K.K. Kartha, 1992. Field evaluation of barley (Hordeumvulgare L.) genotypes derived from tissue culture. Can J Plant Sci 72: 725–733.Google Scholar
  3. Brar, D.S. & S.M. Jain, 1998.Somaclonal variation: mechanism and applications in crop improvement. In: S.M. Jain, D.S. Brar & B.S. Ahloowalia (Eds.), Somaclonal Variation and Induced Mutations in Crop Improvement, pp. 15–37. Kluwer Academic Publishers, Dordrecht.Google Scholar
  4. Bregitzer, P. & M. Poulson, 1995. Agronomic performance of barley lines derived fromtissue culture. Crop Sci 35: 1144–1148.CrossRefGoogle Scholar
  5. Bregitzer, P., M. Poulson & B.L. Jones, 1995. Malting qualityof barley lines derived from tissue culture. Cereal Chem 72: 433–435.Google Scholar
  6. Breiman, A. & D. Rotem-Abarbanell,1990. Somaclonal variation in barley (Hordeum vulgare L.). In: Y.P.S. Bajaj (Ed.), Biotechnology in Agriculture and Forestry, Vol. II. Somaclonal Variation in Crop Improvement I, pp. 352–375. Springer-Verlag, Berlin.Google Scholar
  7. Choo, T.M., J.C. Li, R.A. Martin & K.M. Ho, 2000. AC Malone barley. Can J Plant Sci 80: 597–598.Google Scholar
  8. Dunwell, J.M., M. Cornish, W. Powell & E.M. Borrino, 1986. An evaluation of the field performance of the progeny of plants regenerated from embryos of Hordeum vulgare cv. Golden Promise. J Agric Sci, Camb 107: 561–564.CrossRefGoogle Scholar
  9. Falk, D.E., L.A. Hunt, G. Meatherall & Z. Szlavnics, 1996. Ontario Recommendation Trials: 1995 ProgressReport on Oats, Barley, and Wheat. Department of Crop Science, University of Guelph, Guelph, Ontario.Google Scholar
  10. Fejer, S.O.,G. Fedak & K.M. Ho, 1984. Léger barley. Can J Plant Sci 64: 195–196.CrossRefGoogle Scholar
  11. Gaponenko, A.K.,T.F. Petrova, A.R. Iskakov & A.A. Sozinov, 1988. Cytogenetics of in vitro cultured somatic cells and regenerated plants of barley (Hordeum vulgare L.). Theor Appl Genet 75: 905–911.Google Scholar
  12. Gozukirmizi, N., S. Ari, G. Oraler, Y. Okatan & N. Unsal, 1990. Callus induction, plant regeneration and chromosomal variations in barley. Acta Bot Neerl 39: 379–387.Google Scholar
  13. Hanzel, J.J., J.P. Miller, M.A. Brinkman & E. Fendos, 1985. Genotype and mediaeffects on callus formation and regeneration in barley. Crop Sci 25: 27–31.CrossRefGoogle Scholar
  14. Kaeppler, S.M. & R.L. Phillips,1993. Tissue culture-induced DNA methylation variation in maize. Proc Natl Acad Sci 90: 8773–8776.PubMedCrossRefGoogle Scholar
  15. Karp, A., 1995. Somaclonal variation as a tool for crop improvement. Euphytica 85: 295–302.CrossRefGoogle Scholar
  16. Karp, A.,S.H. Steele, S. Parmar, M.G.K. Jones, P.R. Shewry & A. Breiman, 1987. Relative stability among barley plants regenerated from cultured immature embryos. Genome 29: 405–412.Google Scholar
  17. Luckett, D.J., D. Rose & E. Knights,1989. Paucity of somaclonal variation from immature embryo culture of barley. Aust J Agric Res 40: 1155–1159.CrossRefGoogle Scholar
  18. Lupotto, E., 1984. Callus induction and plant regeneration from mature embryos. Ann Bot 54: 523–529.Google Scholar
  19. Mohanty, B.D. & P.D. Ghosh, 1988. Somatic embryogenesis and plant regeneration from leaf callus ofHordeum vulgare. AnnBot 61: 551–555.Google Scholar
  20. Pickering, R.A., 1989. Plant regeneration and variants from calliderived from immature embryos of diploid barley (Hordeum vulgare L.) and H. vulgare ×H. bulbosum L. crosses. Theor Appl Genet 78: 105–122.CrossRefGoogle Scholar
  21. Steel, R.G.D. & J.H. Torrie, 1980. Principles and Procedures of Statistics.2nd edition. McGraw-Hill Book Company, New York, NY.Google Scholar
  22. Todorovska, E., A. Trifonova, M. Petrova, Z. Vitanova, E. Marinova, M. Gramatikova, D. Valcheva, S. Zaprianov, N. Mersinkov & A. Atanassov, 1997. Agronomic performance and molecular assessment of tissue culture-derived barley lines. Plant Breeding 116: 511–517.CrossRefGoogle Scholar
  23. Ullrich, S.E., J.M. Edmiston, A. Kleinhofs, D.A. Kudrna & M.E.H. Maatougui, 1991. Evaluation ofsomaclonal variation in barley. Cereal Res Comm 19: 245–260.Google Scholar
  24. Veilleux, R.E. & A.A.T. Johnson, 1998.Somaclonal variation: molecular analysis, transformation interaction, and utilization. Plant Breed Reviews 16: 229–268.Google Scholar
  25. Wenzel, G. & B. Foroughi-Wehr, 1990. Progeny tests of barley, wheat, and potato regenerated from cellcultures after in vitro selection for disease resistance. Theor Appl Genet 80: 359–365.CrossRefGoogle Scholar
  26. Yuan, M., Y. Zhang, M. Zhu, A. Xu & H. Pei, 1991. Cytogenetic studies on barley plants regenerated from immature embryo culture and their progenies. Acta Agron Sinica 17: 171–177.Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • J.C. Li
    • 1
  • T.M. Choo
    • 2
  • K.M. Ho
    • 2
  • D.E. Falk
    • 3
  • R. Blatt
    • 4
  1. 1.Biology Institute of ShanxiTaiyuan, Shanxi ProvinceChina
  2. 2.Eastern Cereal and Oilseed Research CentreAgriculture and Agri-Food CanadaOttawaCanada
  3. 3.Department of Crop ScienceUniversity of GuelphGuelphCanada
  4. 4.Atlantic Food and Horticulture Research CentreAgriculture and Agri-Food CanadaKentvilleCanada

Personalised recommendations