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Pharmaceutical Research

, Volume 15, Issue 9, pp 1364–1369 | Cite as

Liposomes Dispersed Within a Thermosensitive Gel: A New Dosage Form for Ocular Delivery of Oligonucleotides

  • Amélie Bochot
  • Elias Fattal
  • Annette Gulik
  • Guy Couarraze
  • Patrick Couvreur
Article

Abstract

Purpose. The main goal of this study was to develop an ocular controlled release formulation of a model oligonucleotide (pdT16), contained within liposomes dispersed within a thermosensitive gel composed by poloxamer 407.

Methods. The influence of the poloxamer concentration 2% or 27% on the stability of the liposomes (PC: CHOL and PC: CHOL: PEG-DSPE) was investigated. The in vitro release profiles of pdT16 from various poloxamer formulations (free pdT16 dispersed within 20% and 27% poloxamer gels, pdT16 encapsulated within liposomes dispersed within 20% and 27% poloxamer gels) were realized using a membrane-free release model.

Results. The dispersion of liposomes within a dilute 2% poloxamer solution resulted in a great leakage of pdT16 from liposomes. However, the destabilization effect of poloxamer was reduced when higher concentration (27%) was used. Poloxamer dissolution was found to control the release process of pdT16, whereas the dispersion of liposomes within 27% poloxamer gel was shown to slow down the diffusion of pdT16 out from the gel.

Conclusions. The dispersion of liposomes within a 27% poloxamer gel presented an interesting system to control the release of a model oligonucleotide compare to a simple gel.

drug delivery system gel dissolution liposomes oligonucleotide poloxamer 407 gels 

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Copyright information

© Plenum Publishing Corporation 1998

Authors and Affiliations

  • Amélie Bochot
    • 1
  • Elias Fattal
    • 1
  • Annette Gulik
    • 2
  • Guy Couarraze
    • 1
  • Patrick Couvreur
    • 1
  1. 1.Laboratoire de Physico-Chimie, Pharmacotechnie, Biopharmacie, URA CNRS 1218, Faculté de PharmacieChâtenay-Malabry CedexFrance
  2. 2.Centre de Génétique MoléculaireFrance

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