Virus Genes

, Volume 23, Issue 2, pp 175–181 | Cite as

Phylogenetic Analysis of Foot-and-Mouth Disease Viruses Isolated in Argentina

  • Guido König
  • Carlos Blanco
  • Nick J. Knowles
  • Eduardo L. Palma
  • Eduardo Maradei
  • Maria Elisa Piccone


We have analysed complete or partial VP1 sequences of 31 foot-and-mouth disease (FMD) viruses belonging to serotypes A, O and C to determine the genetic relatedness of field strains of FMD virus (FMDV) that have circulated in Argentina between 1961 and 1994. Phylogenetic analysis, which also included 15 previously published Argentinean sequences and six reference strains, revealed that (i) FMD type A strains showed the highest genetic heterogeneity and could be divided into five lineages with a sequence divergence of 0.9–18.5% between strains (ii) most of the FMD type O viruses grouped in two clusters (within cluster sequence divergence ranging from 0.2% to 6.0%) circulating in Argentina since the early 1960s, and (iii) FMD type C viruses were grouped in two clusters with a 13.4% nucleotide sequence divergence between each cluster. The availability of sequence data for many more field isolates from the region will enable us to understand the genetic relationships between FMDV strains and to rapidly trace the source of an FMD outbreak for epidemiological surveillance.

Foot-and-mouth disease Molecular epidemiology Phylogenetic analysis Virus evolution 


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  1. 1.
    Huang C.C., Jong M.H., and Lin S.Y., J Vet Med Sci 62(7), 677–679, 2000.Google Scholar
  2. 2.
    Perry B.D., Kalpravidh W., Coleman P.G., Horst H.S., McDermott J.J., Randolph T.F., and Gleeson L.J., Rev Sci Tech Off Int Epiz 18, 478–497, 1999.Google Scholar
  3. 3.
    Pereira H.G., in Gibbs E.P.J. (ed). Virus Disease in Food Animals, vol. 2. Academic Press, New York, pp. 333–363.Google Scholar
  4. 4.
    Bittle J.L., Houghton R.A., Alexander H., Shinnick T.M., Sutcliffe J.G., Lerner R.A., Rowlands D.J., and Brown F., Nature 298, 30–33, 1982.Google Scholar
  5. 5.
    DiMarchi R., Brooke G., Gale C., Cracknell V., Doel T., and Mowat N., Science 232, 639–641, 1982.Google Scholar
  6. 6.
    Piccone M.E., Kaplan G., Giavedoni L., Domingo E., and Palma E.L., J Virol 62, 1469–1473, 1982.Google Scholar
  7. 7.
    Mateu M.G., Camarero J.A., Andreu D., Girald E., and Domingo E., Virology 206, 298–306, 1982.Google Scholar
  8. 8.
    Feigelstock D.A., Mateu M.G., Valero M.L., Andreu D., Domingo E., and Palma E.L., Vaccine 14, 97–102, 1996.Google Scholar
  9. 9.
    Mbayed V., Schiappacassi M., Corominas A., and Campos R., Virus Res 48, 157–163, 1997.Google Scholar
  10. 10.
    Beck E., and Strohmaier K., J Virol 61, 1621–1629, 1987.Google Scholar
  11. 11.
    Knowles N.J., Marquardt O., and Samuel A.R., Rpt Sess Res Grp Stand Tech Comm Eur Comm Cont FMD, Prague, Czechoslovakia. FAO, Rome, Appendix 24, 149–154, 1988.Google Scholar
  12. 12.
    Marquardt O. and Adam K.H., Vet Microbiol 23, 175–183, 1988.Google Scholar
  13. 13.
    Samuel A.R., Knowles N.J., and Kitching R.P., Rpt Sess Res Grp Stand Tech Comm Eur Comm Cont FMD, Denmark, FAO, Rome, pp. 139–145, 1990.Google Scholar
  14. 14.
    Krebs O., Berger H.G., and Marquardt O., Arch Virol 120, 135–143, 1991.Google Scholar
  15. 15.
    Armstrong R.M., Samuel A.R., Knowles N.J., and Uluturk S., Rpt Sess Res Grp Stand Tech Comm Eur Comm Cont FMD, Berne, Switzerland. FAO, Rome, pp. 64–69, 1992.Google Scholar
  16. 16.
    Marquardt O. and Krebs O., Tierartztliche Umschau, 47, 137–140, 1991.Google Scholar
  17. 17.
    Saiz J.C., Sobrino F., and Dopazo J., J Gen Virol 74, 2281–2285, 1991.Google Scholar
  18. 18.
    Samuel A.R., Ansell D.M., Rendell R.T., Armstrong R.M., Davidson F.L., Knowles N.J., and Kitching R.P., Rev Sci Tech Off Int Epiz 12, 839–848, 1993.Google Scholar
  19. 19.
    Stram Y., Chai D., Fawzy H.E., Molad T., Meiri N., Van-Ham M., El-Kilani S., Fahamy F., Moussa A.A., and Yadin H., Arch Virol 140, 1791–1797, 1993.Google Scholar
  20. 20.
    Singh M., Mohan B.M., and Suryanarayan V.V.S., Virus Res 43, 45–55, 1996.Google Scholar
  21. 21.
    Samuel A.R., Knowles N.J., Kitching R.P., and Hafez S.M., Epidem Infect 119, 381–389, 1996.Google Scholar
  22. 22.
    Samuel A.R., Knowles N.J., and Mackay D.K.J., Epidem Infect 122, 529–538, 1999.Google Scholar
  23. 23.
    Knowles N.J., Samuel A.R., Davies P.R., Kitching R.P., Kanno T., Scherbakov A.V., Drygin V.V., Zhao Q.-Z., and Xie Q.-G., Emerging Infectious Diseases, manuscript submitted, 2001.Google Scholar
  24. 24.
    Samuel A.R. and Knowles N.J., J Gen Virol 82, 609–621, 1999.Google Scholar
  25. 25.
    Piccone M.E., Ph D Thesis, University of Buenos Aires 1989.Google Scholar
  26. 26.
    Weddell G., Yansura D.G., Dowbenko D.J., Hoatlin M.E., Grubman M.J., Moore D., and Kleid D.G., Proc Natl Acad Sci USA 82, 2618–2622, 1999.Google Scholar
  27. 27.
    Marquardt O. and Adam K.H., Virus Genes 2, 283–291, 1999.Google Scholar
  28. 28.
    Samuel A.R., Knowles N.J., and Kitching R.P., Epidem Infect 101, 577–590, 1999.Google Scholar
  29. 29.
    Carrillo C., Dopazo J., Moya A., Gonzalez M., Martínez M.A., Saiz J.C., and Sobrino F., Virus Res 15, 45–56, 1999.Google Scholar
  30. 30.
    Armstrong R.M., Samuel A.R., Carpenter W.C., Rama K., and Knowles N.J., Vet Microbiol 39, 285–298, 1999.Google Scholar
  31. 31.
    Martínez M.A., Carrillo C., Plana J., Mascarella R., Bergada J., Palma E.L., Domingo E., and Sobrino F., Gene 62, 75–84, 1988.Google Scholar
  32. 32.
    Sobrino F., Martínez M.A., Carrillo C., and Beck E., Virus Res 14, 273–280, 1988.Google Scholar
  33. 33.
    Knowles N.J. and Samuel A.R., Rpt Sess Res Grp Stand Tech Comm Eur Comm Cont FMD, Lindholm, Denmark. FAO, Rome, Appendix 22, 122–128, 1990.Google Scholar
  34. 34.
    Martínez M.A., Dopazo J., Hernández J., Mateu M.G., Sobrino F., Domingo E., and Knowles N.J., J Virol 66, 3557–3565, 1994.Google Scholar
  35. 35.
    Knowles N.J., and Samuel A.R., Rpt Sess Res Grp Stand Tech Comm Eur Comm Cont FMD, Mödling, Vienna Austria, FAO, Rome, Appendix 8, 45–53, 1995.Google Scholar
  36. 36.
    Felsenstein J., PHYLIP manual version 3.5c University of Washington, Seattle, WA, 1993.Google Scholar
  37. 37.
    Page R.D.M., Computer Applic Biosci 12, 357–358, 1996.Google Scholar
  38. 38.
    Malirat V., Auge de Mello P., Tiraboschi B., Beck E., Gomes I., and Bergmann I.E., Virus Res 34, 31–48, 1994.Google Scholar
  39. 39.
    Boothroyd J.C., Harris T.J.R., Rowlands D.J., and Lowe P.A., Gene 17, 153–161, 1994.Google Scholar
  40. 40.
    Cheung A., Delamarter J., Weiss S., and Kuepper H., J Virol 48, 451–459, 1994.Google Scholar
  41. 41.
    Forss S., Strebel K., Beck E., and Shaller H., Nucl Acids Res 12, 6587–6601, 1994.Google Scholar
  42. 42.
    Rieder Rojas E., Carrillo E., Schiappacassi M., and Campos R., J Virol 66, 3368–3372, 1994.Google Scholar
  43. 43.
    Tami C., Kaplan G., Piccone M.E., and Palma E.L., Virus Genes 14, 255–259, 1994.Google Scholar
  44. 44.
    Mateu M.G., Andreu D., Carreño C., Roig X., Cairo J.J., Camarero J.A., Giralt E., and Domingo E., Eur J Immunol 22, 1385–1389, 1994.Google Scholar
  45. 45.
    Makoff A.J., Paynter C.A., Rowlands D.J., and Boothroyd J.C., Nucl Acids Res 10, 8285–8295, 1994.Google Scholar
  46. 46.
    Mateu M.G., Hernández J., Martínez M.A., Feigelstock D., Lea S., Pérez J.J., Giralt E., Stuart D., Palma E.L., and Domingo E., J Virol 68, 1407–1417, 1994.Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Guido König
    • 1
  • Carlos Blanco
    • 1
  • Nick J. Knowles
    • 2
  • Eduardo L. Palma
    • 1
  • Eduardo Maradei
    • 2
  • Maria Elisa Piccone
    • 1
  1. 1.Instituto de Biotecnologia, INTA, CCBuenos AiresArgentina
  2. 2.UK

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