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Journal of Biomolecular NMR

, Volume 19, Issue 4, pp 367–370 | Cite as

Measurement of 1H3′-31P dipolar couplings in a DNA oligonucleotide by constant-time NOESY difference spectroscopy

  • Zhengrong Wu
  • Nico Tjandra
  • Ad Bax
Article

Abstract

The ratios of cross peak intensities in a selective constant-time NOESY experiment, recorded with and without 31P decoupling, yield values for the sum of the H3′-P scalar and dipolar couplings. The selective refocusing of H3′ resonances in this experiment results in excellent resolution and sensitivity, even in the liquid crystalline phase where the 1H spectrum is broadened by unresolved homonuclear dipolar couplings. The vicinal H3′-P scalar and dipolar couplings in the DNA oligomer d(CGCGAATTCGCG)2 were measured in both isotropic solution, and in a liquid crystalline phase. Isotropic values are in good agreement with values reported previously. Dipolar couplings are in excellent agreement with the NMR structure for this dodecamer, and to a somewhat lesser extent with the X-ray structures.

constant-time NOESY Dickerson dodecamer dipolar coupling liquid crystal Pf1 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Zhengrong Wu
  • Nico Tjandra
  • Ad Bax

There are no affiliations available

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