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Journal of Biomolecular NMR

, Volume 20, Issue 1, pp 77–82 | Cite as

Multiplet component separation for measurement of methyl 13C-1H dipolar couplings in weakly aligned proteins

  • Georg Kontaxis
  • Ad Bax
Article

Abstract

A simple spectral editing procedure is described that generates separate subspectra for the methyl 13C-1H3 multiplet components of 1H-13C HSQC spectra. The editing procedure relies on co-addition of in-phase and antiphase spectra and yields 1H-coupled constant-time HSQC subspectra for the methyl region that have the simplicity of the regular decoupled CT-HSQC spectrum. Resulting spectra permit rapid and reliable measurement of 1H-13C J and dipolar couplings. The editing procedure is illustrated for a Ca2+-calmodulin sample in isotropic and liquid crystalline phases.

dipolar coupling IPAP-editing liquid crystal methyl group multiplet editing 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Georg Kontaxis
  • Ad Bax

There are no affiliations available

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