European Journal of Plant Pathology

, Volume 107, Issue 4, pp 457–465 | Cite as

Cloning, Sequencing and Expression of a Xylanase Gene from the Maize Pathogen Helminthosporium Turcicum

  • Yeshitila Degefu
  • Lars Paulin
  • Peter Stephensen Lübeck
Article

Abstract

A gene encoding an endoxylanase from the phytopathogenic fungus Helminthosporium turcicum Pass. was cloned and sequenced. The entire nucleotide sequence of a 1991 bp genomic fragment containing an endoxylanase gene was determined. The xylanase gene of 795 bp, interrupted by two introns of 52 and 62 bp, encoded a protein of 227 amino acids showing up to 95% amino acid homology with other fungal xylanases. The precise splicing site of the introns was identified by sequencing the corresponding cDNA. A northern blot showed that the gene is expressed when the fungus is grown in a medium containing xylan as a sole carbon source. The cloned xylanase gene was expressed in maize plants during infection.

Endoxylanase Helminthosporium turcicum Zea mays 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Yeshitila Degefu
    • 1
  • Lars Paulin
    • 2
  • Peter Stephensen Lübeck
    • 3
  1. 1.Department of Applied Biology, Plant and Forest PathologyUniversity of HelsinkiHelsinkiFinland
  2. 2.Institute of BiotechnologyUniversity of HelsinkiHelsinkiFinland
  3. 3.Department of MicrobiologyDanish Veterinary LaboratoryCopenhagen V, CopenhagenDenmark

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