Journal of Biomolecular NMR

, Volume 19, Issue 4, pp 361–365 | Cite as

H···N hydrogen bond lengths in double stranded DNA from internucleotide dipolar couplings

  • Zhengrong Wu
  • Akira Ono
  • Masatsune Kainosho
  • Ad Bax


The ratio of the internucleotide dipolar coupling and the corresponding one-bond imino 15N-1H dipolar coupling provides a measure for the N···H/H-N distance ratio. Measurements were carried out for a dodecamer, d(CGCGAATTCGCG)2, in which a C-G and an A-T basepair were uniformly enriched in 15N. When assuming H-bonds to be perfectly linear, dipolar data indicate time-averaged hydrogen bond lengths of 1.80±0.03 Å for A-T and 1.86±0.02 Å for C-G. When using H-bond orientations from high resolution X-ray data, H-bond lengths are about 0.1 Å shorter.

Dickerson dodecamer dipolar coupling E.COSY hydrogen bond liquid crystal Watson–Crick 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Zhengrong Wu
  • Akira Ono
  • Masatsune Kainosho
  • Ad Bax

There are no affiliations available

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