European Journal of Plant Pathology

, Volume 107, Issue 4, pp 421–431 | Cite as

Differential Control of Head Blight Pathogens of Wheat by Fungicides and Consequences for Mycotoxin Contamination of Grain

  • Duncan R. Simpson
  • Gillian E. Weston
  • Judith A. Turner
  • Philip Jennings
  • Paul Nicholson


Fusarium head blight of wheat is caused by a disease complex comprised of toxigenic pathogens, predominantly Fusarium spp., and a non-toxigenic pathogen Microdochium nivale, which causes symptoms visually indistinguishable from Fusarium and is often included as a causal agent of Fusarium head blight. Four field trials are reported here, including both naturally and artificially inoculated trials in which the effect of fungicide treatments were noted on colonisation by Fusarium and Microdochium, and on the production of deoxynivalenol (DON) mycotoxin. The pathogen populations were analysed with quantitative PCR and samples were tested for the presence of the mycotoxin DON. Application of fungicides to reduce Fusarium head blight gave a differential control of these fungi. Tebuconazole selectively controlled F. culmorum and F. avenaceum and reduced levels of DON, but showed little control of M. nivale. Application of azoxystrobin, however, selectively controlled M. nivale and allowed greater colonisation by toxigenic Fusarium species. This treatment also lead to increased levels of DON detected. nobreak Azoxystrobin application two days post-inoculation increased the production of DON mycotoxin per unit of pathogen in an artificially inoculated field trial. This result indicates the potential risk of increased DON contamination of grain following treatment with azoxystrobin to control head blight in susceptible wheat cultivars. This is the first study to show differential fungicidal control of mixed natural pathogen populations and artificial inoculations in field trials.

azoxystrobin fungicide Fusarium Microdochium mycotoxin wheat 


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  1. Bertelsen JR, de-Neergaard E and Smedegaard-Petersem V (1999) Reasons for improved yield when using azoxystrobin in winter wheat. In: 16th Danish Plant Protection Conference. Crop protection in organic farming, Pests and Diseases, Tjele, DenmarkGoogle Scholar
  2. Bottalico A (1998) Fusarium diseases of cereals: species complex and related mycotoxin profiles, in Europe. Journal of Plant Pathology 80: 85–103Google Scholar
  3. D'Mello JPF, Macdonald AMC and Dijksma WTP (1998a) 3-Acetyl deoxynivalenol and esterase production in a fungicide-insensitive strain of Fusarium culmorum. Mycotoxin Research 14: 9–18Google Scholar
  4. D'Mello JPF, Macdonald AMC, Postel D, Dijksma WTP, Dujardin A and Placinta CM (1998b) Pesticide use and mycotoxin production in Fusarium and Aspergillus phytopathogens. European Journal of Plant Pathology 104: 741–751Google Scholar
  5. Desjardins AE and Hohn TM (1997) Mycotoxins in plant pathogenesis. Molecular Plant-Microbe Interactions 10: 147–152Google Scholar
  6. Desjardins AE, Proctor RH, Bai G, McCormick SP, Shaner G, Buechley G and Hohn TM (1996) Reduced virulence of trichothecene-nonproducing mutants of Gibberella zeae in wheat field tests. Molecular Plant-Microbe Interactions 9: 775–781Google Scholar
  7. Doohan FM, Parry DW and Nicholson P (1999) Fusarium ear blight of wheat: the use of quantitative PCR and visual disease assessment in studies of disease control. Plant Pathology 48: 209–217Google Scholar
  8. Doohan FM, Parry DW, Jenkinson P and Nicholson P (1998) The use of species-specific PCR-based assays to analyse Fusarium ear blight of wheat. Plant Pathology 47: 197–205Google Scholar
  9. Faure A and Declercq J (1999) Wheat head blight disease - visible efficacy and grain analysis. Phytoma - La défense des végétaux 517: 12–15Google Scholar
  10. Fehrmann H and Ahrens W (1984) Attack of wheat by Septoria nodorum and Fusarium ear blight II. Spray application of curatively active fungicides. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 2: 113–121Google Scholar
  11. Gareis M and Ceynowa J (1994) Influence of the fungicide Matador (tebuconazole/ triadimenol) on mycotoxin production by Fusarium culmorum. Zeitschrift für Lebensmittel Untersuchung und Forschung 198: 244–248Google Scholar
  12. Golinski P, Kostecki M, Lasocka I, Wisniewska H, Chelkowski J and Kaczmarek Z (1996) Moniliformin accumulation and other effects of Fusarium avenaceum (Fr.) Sacc. On kernels of winter wheat cultivars. Journal of Phytopathology 144: 495–499Google Scholar
  13. González HHL, Martínez EJ, Pacin Aand Resnik SL (1999) Relationship between Fusarium graminearum and Alternaria alternata contamination and deoxynivalenol occurrence on Argentinian durum wheat. Mycopathologia 144: 97–102Google Scholar
  14. Hardy A, Silva-Fernandes A, Speijers G, Hans R, Delcour M-P, Kuiper H, Führ F, Carere A, Richard-Molard D and Thomas M (1999) Opinion on the relationship between the use of plant protection products on food plants and the occurrence of mycotoxins in foods. Scientific Committee on Plants. European Commission Health and Consumer Protection Directorate-General SCP/RESI/063-Final, Brussels, BelgiumGoogle Scholar
  15. Herrmann M, Zocher R and Haese A (1996) Effect of disruption of the enniatin synthetase gene on the virulence of Fusarium avenaceum. Molecular Plant-Microbe Interactions 9: 226–232Google Scholar
  16. Hopwood A, Oldroyd N, Fellows S, Ward R, Owen S-A and Sullivan K (1997) Rapid quantification of DNA samples extracted from buccal scrapes prior to DNA profiling. Biotechniques 23: 18–20Google Scholar
  17. Hutcheon JA and Jordan VWL (1992) Fungicide timing and performance for Fusarium control in wheat. Brighton Crop Protection Conference - Pests and Diseases 633–638Google Scholar
  18. Langseth W, Bernhoft A, Rundberget T, Kosiak B and Gareis N (1998) Mycotoxin production and cytotoxicity of Fusarium strains isolated from Norwigian cereals. Mycopathologia 144: 103–113Google Scholar
  19. Liggitt J, Jenkinson P and Parry DW (1997) The role of saprophytic microflora in the development of Fusarium ear blight of winter wheat caused by Fusarium culmorum. Crop Protection 16: 679–685Google Scholar
  20. Logrieco A, Vesonder RF, Peterson SW and Bottalico A (1991) Reexamination of the taxonomic disposition of and deoxynivalenol production by Fusarium nivale NRRL 3289. Mycologia 83: 367–370Google Scholar
  21. McMullen M, Jones R and Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease or devastating impact. Plant Disease 81: 1340–1348Google Scholar
  22. Martin RA and Johnston HW (1982) Effects and control of Fusarium diseases of cereal grains in the Atlantic Provinces. Canadian Journal of Plant Pathology 4: 210–216Google Scholar
  23. Matthies A and Buchenauer H (2000) Effect of tebuconazole (Folicur®) and prochloraz (Sportak®) treatments on Fusarium head scab development, yield and deoxynivalenol (DON) content in grains of wheat following artificial inoculation with Fusarium culmorum. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 107: 33–52Google Scholar
  24. Matthies A, Walker F and Buchenaur H (1999) Interference of selected fungicides, plant growth retardants as well as piperonyl butoxide and 1-aminobenzotriazole in trichothecene production of Fusarium graminearum (strain 4528) in vitro. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 106: 198–212Google Scholar
  25. Mauler-Machnik A and Suty A (1997) New findings on the epidemiology, importance and control of Fusarium ear blight on wheat. Cereal Research Communications 25: 705–709Google Scholar
  26. Mercer PC and Ruddock A (1998) Evaluation of azoxystrobin and a range of conventional fungicides on yield, Septoria tritici and senescence in winter wheat. Tests of Agrochemicals and Cultivars No. 19 Annals of Applied Biology 132 (Supplement)Google Scholar
  27. Mesterházy A and Bartók T (1997) Effect of chemical control on FHB and toxin contamination of wheat. Cereal Research Communications 25: 781–783Google Scholar
  28. Mesterházy A, Bartók T, Mirocha CG and Komoróczy R (1999) Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breeding 118: 97–110Google Scholar
  29. Miller JD (1994) Epidemiology of Fusarium ear diseases of cereals. In: Miller JD and Trenholm HL (eds) Mycotoxins in Grain, Compounds Other Than Aflatoxin. (pp 19–36) Egan Press, MN, USAGoogle Scholar
  30. Milus EA and Parsons CE (1994) Evaluation of foliar fungicides for controlling Fusarium head blight of wheat. Plant Disease 78: 697–699Google Scholar
  31. Moss MO and Frank JM (1985) Influence of the fungicide tridemorph on t-2 toxin production by Fusarium sporotrichoides. Transactions of the British Mycological Society 84: 585–590Google Scholar
  32. Nakajima T and Abe J (1990) A method for assessing resistance to snow molds Typhula incarnata and Microdochium nivale in winter wheat incubated at the optimum growth temperature ranges of the fungi. Canadian Journal of Botany 68: 343–346Google Scholar
  33. Nicholson P and Parry DW(1996) Development and use of a PCR assay to detect Rhizoctonia cerealis, the cause of sharp eyespot in wheat. Plant Pathology 45: 872–883Google Scholar
  34. Nicholson P, Lees AK, Maurin N, Parry DW and Rezanoor HN (1996) Development of a PCR assay to identify and quantify Microdochium nivale var. nivale and Microdochium nivale var. majus in wheat. Physiological and Molecular Plant Pathology 48: 257–271Google Scholar
  35. Nicholson P, Simpson DR, Weston GE, Rezanoor HN, Lees AK, Parry DW and Joyce D (1998) Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiological and Molecular Plant Pathology 53: 17–37Google Scholar
  36. Nirenberg HI (1981) A simplified method for identifying Fusarium spp. occurring on wheat. Canadian Journal of Botany 59: 1599–1609Google Scholar
  37. Parry DW and Nicholson P (1996) Development of a PCR assay to detect Fusarium poae in wheat. Plant Pathology 45: 383–391Google Scholar
  38. Parry DW, Jenkinson P and McLeod L (1995a) Fusarium ear blight (scab) in small grain cereals - a review. Plant Pathology 44: 207–238Google Scholar
  39. Parry DW, Rezanoor HN, Pettitt TR, Hare MC and Nicholson P (1995b) Analysis of Microdochium nivale isolates from wheat in the UK during 1993. Annals of Applied Biology 126: 449–455Google Scholar
  40. Proctor RH, Hohn TM and McCormick SP (1995) Reduced virulence of Gibberella zeae caused by disruption of a trichothecene toxin biosynthetic gene. Molecular Plant-Microbe Interactions 8: 593–601Google Scholar
  41. Reinecke P, Duben J and Fehrmann H (1979) Antagonism between fungi of the foot rot complex of cereals. In: Schippers B and Gams W (eds) Soil-Borne Plant Pathogens (pp 327–336) Academic Press, LondonGoogle Scholar
  42. Simpson DR, Rezanoor HN, Parry DW and Nicholson P (2000) Evidence for differential host preference in Microdochium nivale var. majus and Microdochium nivale var. nivale. Plant Pathology 49: 261–268Google Scholar
  43. Steel RGD, Torrie JH (1981) Principles and procedures of statistics: a biometrical approach. 2nd ed McGraw-Hill, LondonGoogle Scholar
  44. Turner JA, Jennings P and Nicholson P (1999) Investigation of Fusarium infection and mycotoxin levels in harvested wheat grain (1998). HGCA Project Report No 207. HGCA, LondonGoogle Scholar
  45. Turner AS, Lees AK, Rezanoor HN and Nicholson P (1998) Refinement of PCR-detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum. Plant Pathology 47: 278–288Google Scholar
  46. Zadoks JC, Chang TT and Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Research 14: 415–421Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Duncan R. Simpson
    • 1
  • Gillian E. Weston
    • 1
  • Judith A. Turner
    • 2
  • Philip Jennings
    • 2
  • Paul Nicholson
    • 3
  1. 1.John Innes CentreNorwichUK
  2. 2.Central Science Laboratory, Sand HuttonYorkUK
  3. 3.John Innes CentreNorwichUK

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