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Journal of Biomolecular NMR

, Volume 20, Issue 4, pp 379–384 | Cite as

Amino acid type-selective backbone 1H-15N-correlations for Arg and Lys

  • Mario Schubert
  • Hartmut Oschkinat
  • Peter Schmieder
Article

Abstract

Four novel amino acid type-selective triple resonance experiments to identify the backbone amino proton and nitrogen resonances of Arg and Lys and of their sequential neighbors in (13C,15N)-labeled proteins are presented: the R(i+1)-HSQC and R(i,i+1)-HSQC select signals originating from Arg side chains, the K(i+1)-HSQC and K(i,i+1)-HSQC select signals originating from Lys side chains. The selection is based on exploiting the characteristic chemical shifts of a pair of carbon atoms in Arg and Lys side chains using selective 90° pulses. The new experiments are recorded as two-dimensional 1H-15N-correlations and their performance is demonstrated with the application to a protein domain of 83 amino acids.

amino acid type-selective experiments automated assignment HSQC proteins triple-resonance 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Mario Schubert
    • 1
  • Hartmut Oschkinat
    • 1
  • Peter Schmieder
    • 1
  1. 1.Forschungsinstitut für Molekulare PharmakologieBerlinGermany

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