A Practical Guide for the Determination of Binding Constants

  • Keiji Hirose
Article

Abstract

When working in the field ofhost–guest chemistry, the binding constants have to bedetermined on many occasions. Here is a detaileddocument of how to determine the binding constantswhich covers both the basic principle and thepractical issue: a practical experimental guideline,a representative method for the determination ofstoichiometry and for the evaluation of a complexconcentration, precautions to be taken on setting upconcentration conditions of the titration experiment,practical data-treatment methods and estimation ofstatistical errors. This document is described indetail using the basic level of mathematics,statistics, and programs of spreadsheet software.Especially, the titration experiments by means ofUV-visible and NMR spectroscopy are carried out anddescribed.

binding constant association constant stability constant UV-visible spectroscopy NMR Rose–Drago method non-linear least squares method supramolecule host–guest complexation 

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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Keiji Hirose
    • 1
  1. 1.Department of Chemistry, Faculty of Engineering ScienceOsaka UniversityToyonaka, OsakaJapan

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